CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A
- 8 November 2011
- journal article
- Published by Oxford University Press (OUP) in FEMS Immunology & Medical Microbiology
- Vol. 64 (2), 169-174
- https://doi.org/10.1111/j.1574-695x.2011.00874.x
Abstract
Staphylococcus aureus is a major bacterial pathogen producing a group of 21 enterotoxins (SEs). These enterotoxins have two separate but related biological activities. They cause gastroenteritis, and they function as superantigens that activate large numbers of T cells. In the current study, we demonstrate that short-term ex vivo exposure of primary naïve CD4+ T-cells to SEA induces differential expression of the T cell surface receptor CD154 in a time- and dose-dependent manner. In addition, we show that SEA induces higher CD154 protein expression and higher splenocyte cell proliferation compared with SEB. We also demonstrate that expression of CD154 can be used for rapid detection of active SEA in milk.Keywords
This publication has 16 references indexed in Scilit:
- Immunization with a Nontoxic Mutant of Staphylococcal Enterotoxin A, SEAD227A, Protects against Enterotoxin‐Induced Emesis in House Musk ShrewsThe Journal of Infectious Diseases, 2009
- Protein Standard Absolute Quantification (PSAQ) for improved investigation of staphylococcal food poisoning outbreaksProteomics, 2008
- Mouse γδ T cells are capable of expressing MHC class II molecules, and of functioning as antigen-presenting cellsJournal of Neuroimmunology, 2008
- Staphylococcus aureus enterotoxin C2 mutants: biological activity assay in vitroJournal of Industrial Microbiology & Biotechnology, 2008
- Staphylococcal Enterotoxin and Its Rapid Identification in Foods by Enzyme-Linked Immunosorbent Assay–Based MethodologyJournal of Food Protection, 2005
- Other biologic toxin bioweapons: ricin, staphylococcal enterotoxin B, and trichothecene mycotoxinsDermatologic Clinics, 2004
- An extensive outbreak of staphylococcal food poisoning due to low-fat milk in Japan: estimation of enterotoxin A in the incriminated milk and powdered skim milkEpidemiology and Infection, 2003
- Staphylococcal enterotoxinsInternational Journal of Food Microbiology, 2000
- Exotoxins of Staphylococcus aureusClinical Microbiology Reviews, 2000
- [45] Monkey feeding test for staphylococcal enterotoxinMethods in Enzymology, 1988