Immunization with a Nontoxic Mutant of Staphylococcal Enterotoxin A, SEAD227A, Protects against Enterotoxin‐Induced Emesis in House Musk Shrews
Open Access
- 1 February 2009
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 199 (3), 302-310
- https://doi.org/10.1086/596065
Abstract
Background. Staphylococcal enterotoxins (SEs) are the most common cause of foodborne diseases and toxic shock throughout the world. However, no vaccine that prevents emesis induced by SEs has been described. Methods. A nontoxic mutant of SEA, SEAD227A, was constructed by site-directed mutagenesis and was purified by means of the Escherichia coli expression system. House musk shrews, a small emetic animal model, were immunized with SEAD227A and then challenged with wild-type SEA. SEA-induced emesis was recorded for 3 h. Antibody production was analyzed by gel double-immunodiffusion assay. Neutralizing activities of the antibodies with respect to superantigenic and emetic activities were analyzed in vitro and in vivo. Results. SEAD227A was devoid of both superantigenic and emetic activities but still retained its immunological activity. Immunization with SEAD227A strongly induced specific antibody production and provided significant protection against SEA-induced emesis. Antibodies from immunized shrews markedly inhibited the SEA-induced proliferation of spleen cells and also significantly ablated SEA-induced vomiting in the animals. Conclusions. These results suggest that vaccination with SEAD227A, which is devoid of toxic properties, provides protection against SEA-induced emesis. This nontoxic mutant and its specific antibodies might be useful in the prevention and treatment of staphylococcal food poisoning.Keywords
This publication has 32 references indexed in Scilit:
- Staphylococcal Enterotoxin-Like Toxins U2 and V, Two New Staphylococcal Superantigens Arising from Recombination within the Enterotoxin Gene ClusterInfection and Immunity, 2006
- A Case Study of a Massive Staphylococcal Food Poisoning IncidentFoodborne Pathogens & Disease, 2004
- Biological Properties of Staphylococcal Enterotoxin-Like Toxin Type RInfection and Immunity, 2004
- Exotoxins ofStaphylococcus aureusClinical Microbiology Reviews, 2000
- Development of engineered vaccines effective against structurally related bacterial superantigensVaccine, 1998
- A structural and functional comparison of staphylococcal enterotoxins A and C2 reveals remarkable similarity and dissimilarityJournal of Molecular Biology, 1997
- Bacterial pyrogenic exotoxins as superantigensClinical Microbiology Reviews, 1995
- T-cell antigen receptor binding sites for the microbial superantigen staphylococcal enterotoxin A.Proceedings of the National Academy of Sciences of the United States of America, 1992
- In vivo responses of CD4+ and CD8+ cells to bacterial superantigensEuropean Journal of Immunology, 1992
- Staphylococcal and Streptococcal Pyrogenic Toxins Involved in Toxic Shock Syndrome and Related IllnessesCritical Reviews in Microbiology, 1990