An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
Open Access
- 1 January 2008
- journal article
- Published by Springer Science and Business Media LLC in Virology Journal
- Vol. 5 (1), 152
- https://doi.org/10.1186/1743-422x-5-152
Abstract
To perform a retrospective analysis of patients with IgM antibodies to dengue fever infection to determine the serotypes present by molecular techniques. A representative sample (~20%/per year) of patients diagnosed with dengue fever infection were selected based on the detection of IgM antibodies in the acute phase serum sample. RNA was extracted from each sample and reverse transcribed. Following this, the amplicons were electrophoresed and serotyped based on band sizes.Keywords
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