Chemical Composition andIn VitroCytotoxic Activity of Essential Oil of Leaves ofMalus domesticaGrowing in Western Himalaya (India)
Open Access
- 29 April 2012
- journal article
- research article
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 2012, 1-6
- https://doi.org/10.1155/2012/649727
Abstract
Light pale-colored volatile oil was obtained from fresh leaves ofMalus domesticatree, growing in Dhauladhar range of Himalaya (Himachal Pradesh, India), with characteristic eucalyptol dominant fragrance. The oil was found to be a complex mixture of mono-, sesqui-, di-terpenes, phenolics, and aliphatic hydrocarbons. Seventeen compounds accounting for nearly 95.3% of the oil were characterized with the help of capillary GC, GC-MS, and NMR. Major compounds of the oil were characterized as eucalyptol (43.7%), phytol (11.5%), α-farnesene (9.6%), and pentacosane (7.6%). Cytotoxicity of essential oil of leaves ofM. domesticawas evaluated by sulforhodamine B (SRB) assays. The essential oil of leaves ofM. domestica, tested against three cancer cell lines, namely, C-6 (glioma cells), A549 (human lung carcinoma), CHOK1 (Chinese hamster ovary cells), and THP-1 (human acute monocytic leukemia cell). The highest activity showed by essential oil on C-6 cell lines (98.2%) at concentration of 2000 μg/ml compared to control. It is the first paper in literature to exploit the chemical composition and cytotoxic activity of leaves essential oil ofM. domestica.Keywords
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