Hypercapnic vasodilatation in isolated rat basilar arteries is exerted via low pH and does not involve nitric oxide synthase stimulation or cyclic GMP production
- 8 December 1994
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 152 (4), 391-397
- https://doi.org/10.1111/j.1748-1716.1994.tb09821.x
Abstract
The relaxant effect of hypercapnia (15% CO2) was studied in isolated circular segments of rat basilar arteries with intact endothelium. The nitric oxide synthase inhibitor nitro‐l‐arginine (l‐NOARG) and the cytosolic guanylate cyclase inhibitor methylene blue (MB), significantly reduced this relaxation by 54% and 70%, respectively. The effect of l‐NOARG was completely reversed by l‐arginine. Blockade of nerve excitation with tetrodotoxin (TTX) had no affect on the 15% CO2 elicited vasodilatation. Measurements of cGMP in vessel segments showed no significant increase in cGMP content in response to hypercapnia. l‐NOARG and MB, but not TTX, significantly reduced the basal cGMP content in cerebral vessels. Adding 1.5% halothane to the incubation medium did not result in a significant increase in cGMP content. Lowering the pH by cumulative application of 0.12 m HCl resulted in relaxation identical to that obtained by lowering the pH with 15% CO2. In vessel segments in which the endothelium had been removed beforehand 15% CO2 induced relaxation that was not different from that seen in vessels with intact endothelium. l‐NOARG had no affect in endothelium denuded vessels. The results suggest that high CO2 elicits vasodilatation of isolated rat basilar arteries by a mechanism independent of nitric oxide synthase (NOS) activity. The markedly reduced basal cGMP levels in cerebral vessels by l‐NOARG and MB suggest that there exists a basal NO formation in the cerebral vessel wall.Keywords
This publication has 31 references indexed in Scilit:
- Relation between Cyclic GMP Generation and Cerebrovascular Reactivity: Modulation by NPY and α-TrinositolBasic & Clinical Pharmacology & Toxicology, 1995
- Endothelium-independent contractions to NG-monomethyl-L-arginine in canine basilar artery.Stroke, 1991
- Chronic Hyperglycemic Diabetes in the Rat is Associated with a Selective Impairment of Cerebral Vasodilatory ResponsesJournal of Cerebral Blood Flow & Metabolism, 1991
- Interleukin-1 β induces the production of an L-arginine-derived relaxing factor from cultured smooth muscle cells from rat aortaBiochemical and Biophysical Research Communications, 1991
- Dexamethasone prevents the induction by endotoxin of a nitric oxide synthase and the associated effects on vascular tone: An insight into endotoxin shockBiochemical and Biophysical Research Communications, 1990
- Induction of nitric oxide synthase by cytokines in vascular smooth muscle cellsFEBS Letters, 1990
- Localization of nitric oxide synthase indicating a neural role for nitric oxideNature, 1990
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980
- Analysis of vasoactivity of local pH, PCO2 and bicarbonate on pial vessels.Stroke, 1977
- Local mechanism of CO2 action of cat pial arterioles.Stroke, 1977