Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells
Open Access
- 1 March 2008
- journal article
- Published by American Society of Hematology in Blood
- Vol. 111 (5), 2631-2635
- https://doi.org/10.1182/blood-2007-07-099622
Abstract
We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units–fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies.Keywords
This publication has 23 references indexed in Scilit:
- Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCsBlood, 2007
- SSEA-4 identifies mesenchymal stem cells from bone marrowPublished by American Society of Hematology ,2006
- Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartmentBlood, 2006
- Characterization of nonexpanded mesenchymal progenitor cells from normal adult human bone marrowExperimental Hematology, 2005
- STRO-1, HOP-26 (CD63), CD49a and SB-10 (CD166) as markers of primitive human marrow stromal cells and their more differentiated progeny: a comparative investigation in vitroCell and tissue research, 2003
- Perivascular Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental PulpJournal of Bone and Mineral Research, 2003
- Pluripotency of mesenchymal stem cells derived from adult marrowNature, 2002
- Multilineage Potential of Adult Human Mesenchymal Stem CellsScience, 1999
- MUC18, a member of the immunoglobulin superfamily, is expressed on bone marrow fibroblasts and a subset of hematological malignanciesLeukemia, 1998
- THE DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA‐PIG BONE MARROW AND SPLEEN CELLSCell Proliferation, 1970