Perivascular Niche of Postnatal Mesenchymal Stem Cells in Human Bone Marrow and Dental Pulp
Top Cited Papers
Open Access
- 1 April 2003
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 18 (4), 696-704
- https://doi.org/10.1359/jbmr.2003.18.4.696
Abstract
Mesenchymal stem cell populations have previously been identified in adult bone marrow and dental pulp that are capable of regenerating the bone marrow and dental pulp microenvironments, respectively. Here we show that these stem cell populations reside in the microvasculature of their tissue of origin. Human bone marrow stromal stem cells (BMSSCs) and dental pulp stem cells (DPSCs) were isolated by immunoselection using the antibody, STRO-1, which recognizes an antigen on perivascular cells in bone marrow and dental pulp tissue. Freshly isolated STRO-1 positive BMSSCs and DPSCs were tested for expression of vascular antigens known to be expressed by endothelial cells (von Willebrand factor, CD146), smooth muscle cells, and pericytes (α-smooth muscle actin, CD146), and a pericyte-associated antigen (3G5), by immunohistochemistry, fluorescence-activated cell sorting (FACS), and/or immunomagnetic bead selection. Both BMSSCs and DPSCs lacked expression of von Willebrand factor but were found to be positive for α-smooth muscle actin and CD146. Furthermore, the majority of DPSCs expressed the pericyte marker, 3G5, while only a minor population of BMSSCs were found to be positive for 3G5. The finding that BMSSCs and DPSCs both display phenotypes consistent with different perivascular cell populations, regardless of their diverse ontogeny and developmental potentials, may have further implications in understanding the factors that regulate the formation of mineralized matrices and other associated connective tissues.Keywords
This publication has 52 references indexed in Scilit:
- Adult rat and human bone marrow stromal cells differentiate into neuronsJournal of Neuroscience Research, 2000
- Stem CellsCell, 2000
- Uno, nessuno e centomila: Searching for the Identity of Mesodermal ProgenitorsExperimental Cell Research, 1999
- Multilineage Potential of Adult Human Mesenchymal Stem CellsScience, 1999
- Isolation, purification and in vitro manipulation of human bone marrow stromal precursor cellsPublished by Cambridge University Press (CUP) ,1998
- MUC18, a member of the immunoglobulin superfamily, is expressed on bone marrow fibroblasts and a subset of hematological malignanciesLeukemia, 1998
- Single-Colony Derived Strains of Human Marrow Stromal Fibroblasts Form Bone After Transplantation In VivoJournal of Bone and Mineral Research, 1997
- A monoclonal antibody (3G5)-defined ganglioside antigen is expressed on the cell surface of microvascular pericytes.The Journal of Experimental Medicine, 1988
- The regulation of haemopoiesis in long‐term bone marrow cultures: I. Role of L‐cell CSFJournal of Cellular Physiology, 1980
- STROMAL CELLS RESPONSIBLE FOR TRANSFERRING THE MICROENVIRONMENT OF THE HEMOPOIETIC TISSUESTransplantation, 1974