Fine mapping of dental fluorosis quantitative trait loci in mice
- 1 December 2011
- journal article
- Published by Wiley in European Journal of Oral Sciences
- Vol. 119 (s1), 8-12
- https://doi.org/10.1111/j.1600-0722.2011.00868.x
Abstract
Everett ET, Yin Z, Yan D, Zou F. Fine mapping of dental fluorosis quantitative trait loci in mice. Eur J Oral Sci 2011; 119 (Suppl. 1): 8–12. © 2011 Eur J Oral Sci Genetic factors underlie the susceptibility and the resistance to dental fluorosis (DF). The A/J (DF susceptible) and 129P3/J (DF resistant) mouse strains have previously been used to detect quantitative trait loci (QTLs) associated with DF on chromosome (Chr) 2 and Chr 11. In the present study, increased marker density genotyping followed by interval mapping was performed to narrow the QTL intervals and improve the logarithm of the odds (to the base 10) (LOD) scores. Narrower intervals were obtained on Chr 2 where LOD ≥ 6.0 (57–84 cM or ∼51 Mb), LOD ≥ 7.0 (62–79 cM or ∼32 Mb), and LOD ≥ 8.0 (65–74 cM or ∼17 Mb); and on Chr 11 where LOD ≥ 6.0 (18–51 cM or ∼53 Mb), LOD ≥ 7.0 (28–48 cM or ∼34 Mb), and LOD ≥ 8.0 (31–45 cM or∼22 Mb). Haplotype analysis between A/J and 129P3/J mice further reduced the QTL intervals. Accn1 was selected as a candidate gene based upon its location near the peak LOD score on Chr 11 and distant homology with the Caenorhabditis elegans fluoride‐resistance gene, flr1. The severity of DF between Accn1−/− and wild‐type mice was not significantly different. Hence, the loss of ACCN1 function does not modify DF severity in mice. Narrowing the DF QTL intervals will facilitate additional candidate gene selections and interrogation.Keywords
This publication has 28 references indexed in Scilit:
- Subspecific origin and haplotype diversity in the laboratory mouseNature Genetics, 2011
- Phenotypic Variation of Fluoride Responses between Inbred Strains of MiceCells Tissues Organs, 2011
- Fluoride’s Effects on the Formation of Teeth and Bones, and the Influence of GeneticsJournal of Dental Research, 2010
- The Ion Channel ASIC2 Is Required for Baroreceptor and Autonomic Control of the CirculationNeuron, 2009
- The Impact of Fluoride on Ameloblasts and the Mechanisms of Enamel FluorosisJournal of Dental Research, 2009
- A New Standard Genetic Map for the Laboratory MouseGenetics, 2009
- Fluoride effects on bone formation and mineralization are influenced by geneticsBone, 2008
- Detection of Dental Fluorosis-Associated Quantitative Trait Loci on Mouse Chromosomes 2 and 11Cells Tissues Organs, 2008
- Genetic background influences fluoride's effects on osteoclastogenesisBone, 2007
- Fine-mapping of a QTL influencing pork tenderness on porcine chromosome 2BMC Genomic Data, 2007