Resident Microbiota of the Gypsy Moth Midgut Harbors Antibiotic Resistance Determinants
- 1 March 2009
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA and Cell Biology
- Vol. 28 (3), 109-117
- https://doi.org/10.1089/dna.2008.0812
Abstract
Little is known about the significance of insects as environmental reservoirs of antibiotic-resistant bacteria. We characterized the antibiotic resistome of the microbial community in gypsy moth larval midguts by applying functional metagenomics to cultured isolates. The minimum inhibitory concentrations of 12 antibiotics were determined for 44 cultured isolates, and antibiotic resistance genes were selected from metagenomic libraries derived from DNA extracted from a pool of the isolates. Six unique clones were identified. Two were highly resistant to penicillin-type β-lactams, two were moderately resistant to erythromycin, and two were moderately resistant to a range of antibiotics, including erythromycin, carbenicillin, and chloramphenicol. Sequence analysis predicted that the active genes encoded efflux pumps, a transcriptional activator of efflux pump protein expression, and an extended-spectrum class A β-lactamase. Insect guts are a reservoir of antibiotic resistance genes with the potential for dissemination.Keywords
This publication has 38 references indexed in Scilit:
- The continuing challenge of ESBLsCurrent Opinion in Pharmacology, 2007
- β-Lactam resistance and β-lactamases in bacteria of animal originVeterinary Microbiology, 2007
- Ecology of Antibiotic Resistance Genes: Characterization of Enterococci from Houseflies Collected in Food SettingsApplied and Environmental Microbiology, 2006
- Sampling the Antibiotic ResistomeScience, 2006
- ProtTest: selection of best-fit models of protein evolutionBioinformatics, 2005
- Salmonella entericaSerovar Typhimurium RamA, Intracellular Oxidative Stress Response, and Bacterial VirulenceInfection and Immunity, 2004
- Growing Group of Extended-Spectrum β-Lactamases: the CTX-M EnzymesAntimicrobial Agents and Chemotherapy, 2004
- Identification of a Chromosome-Borne Expanded-Spectrum Class A β-Lactamase from Erwinia persicinaAntimicrobial Agents and Chemotherapy, 2002
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Basic local alignment search toolJournal of Molecular Biology, 1990