Tetracationic Bis-Triarylborane 1,3-Butadiyne as a Combined Fluorimetric and Raman Probe for Simultaneous and Selective Sensing of Various DNA, RNA, and Proteins
Open Access
- 12 May 2020
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 26 (27), 6017-6028
- https://doi.org/10.1002/chem.201905328
Abstract
A bis-triarylborane tetracation (4-Ar2B-3,5-Me2C6H2)-C equivalent to C-C equivalent to C-(3,5-Me2C6H2-4-BAr2 [Ar=(2,6-Me-2-4-NMe3-C6H2)(+)] (2(4+)) shows distinctly different behaviour in its fluorimetric response than that of our recently published bis-triarylborane 5-(4-Ar2B-3,5-Me2C6H2)-2,2 '-(C4H2S)(2)-5 '-(3,5-Me2C6H2-4-BAr2) (3(4+)). Single-crystal X-ray diffraction data on the neutral bis-triarylborane precursor 2 N confirm its rod-like dumbbell structure, which is shown to be important for DNA/RNA targeting and also for BSA protein binding. Fluorimetric titrations with DNA/RNA/BSA revealed the very strong affinity of 2(4+) and indicated the importance of the properties of the linker connecting the two triarylboranes. Using the butadiyne rather than a bithiophene linker resulted in an opposite emission effect (quenching vs. enhancement), and 2(4+) bound to BSA 100 times stronger than 3(4+). Moreover, 2(4+) interacted strongly with ss-RNA, and circular dichroism (CD) results suggest ss-RNA chain-wrapping around the rod-like bis-triarylborane dumbbell structure like a thread around a spindle, a very unusual mode of binding of ss-RNA with small molecules. Furthermore, 2(4+) yielded strong Raman/SERS signals, allowing DNA or protein detection at ca. 10 nm concentrations. The above observations, combined with low cytotoxicity, efficient human cell uptake and organelle-selective accumulation make such compounds intriguing novel lead structures for bio-oriented, dual fluorescence/Raman-based applications.Funding Information
- Hrvatska Zaklada za Znanost (IP-2018-01-5475)
- Deutscher Akademischer Austauschdienst
- Julius-Maximilians-Universität Würzburg
- Alexander von Humboldt-Stiftung
This publication has 89 references indexed in Scilit:
- Exploiting the Nanoparticle Plasmon Effect: Observing Drug Delivery Dynamics in Single Cells via Raman/Fluorescence Imaging SpectroscopyACS Nano, 2013
- Synthesis of 2‐ and 2,7‐Functionalized Pyrene Derivatives: An Application of Selective C-H BorylationChemistry – A European Journal, 2012
- Multimodal spectroscopy detects features of vulnerable atherosclerotic plaqueJournal of Biomedical Optics, 2011
- A multimodal spectroscopy system for real-time disease diagnosisReview of Scientific Instruments, 2009
- Infrared and Raman spectra of ethyl trifluoromethanesulfonate, CF3SO2OCH2CH3. An experimental and theoretical studyJournal of Raman Spectroscopy, 2005
- The Conversion of Phenols to the Corresponding Aryl Halides Under Mild ConditionsSynthesis, 2005
- Preparation and Properties of Phosphaethynes Bearing Bulky Aryl Groups with Electron-Donating Substituents at the Para PositionThe Journal of Organic Chemistry, 2004
- Analysis of Thermal Melting CurvesOligonucleotides, 2003
- Theoretical aspects of DNA-protein interactions: Co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous latticeJournal of Molecular Biology, 1974
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949