Analysis of Thermal Melting Curves
Top Cited Papers
- 1 December 2003
- journal article
- review article
- Published by Mary Ann Liebert Inc in Oligonucleotides
- Vol. 13 (6), 515-537
- https://doi.org/10.1089/154545703322860825
Abstract
Tm is defined as Temperature of melting or, more accurately, as temperature of midtransition. This term is often used for nucleic acids (DNA and RNA, oligonucleotides and polynucleotides). A thermal denaturation experiment determines the stability of the secondary structure of a DNA or RNA and aids in the choice of the sequences for antisense oligomers or PCR primers. Beyond a simple numerical value (the Tm), a thermal denaturation experiment, in which the folded fraction of a structure is plotted vs. temperature, yields important thermodynamic information. We present the classic problems encountered during these experiments and try to demonstrate that a number of useful pieces of information can be extracted from these experimental curves.Keywords
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