Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase
- 1 October 1978
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 275 (5681), 617-624
- https://doi.org/10.1038/275617a0
Abstract
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.This publication has 30 references indexed in Scilit:
- A bacterial clone synthesizing proinsulin.Proceedings of the National Academy of Sciences of the United States of America, 1978
- A sensitive radioimmunoassay for detecting products translated from cloned DNA fragmentsCell, 1978
- Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone SomatostatinScience, 1977
- Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassaProceedings of the National Academy of Sciences of the United States of America, 1977
- Functional expression of cloned yeast DNA in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Functional genetic expression of eukaryotic DNA in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America, 1976
- The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.Nucleic Acids Research, 1976
- Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.Proceedings of the National Academy of Sciences of the United States of America, 1975
- Genetic studies with heteroduplex DNA of bacteriophage f1. Asymmetric segregation, base correction and implications for the mechanism of genetic recombinationJournal of Molecular Biology, 1975
- Replication and Transcription of Eukaryotic DNA in Esherichia coliProceedings of the National Academy of Sciences of the United States of America, 1974