Construction of Human Gene Libraries from Small Amounts cf Peripheral Blood: Analysis of β-Like Globin Genes
- 1 January 1982
- journal article
- Published by Informa UK Limited in Hemoglobin
- Vol. 6 (1), 27-36
- https://doi.org/10.3109/03630268208996930
Abstract
We describe a rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood. High molecular weight DNA is isolated from 5–20 ml peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using bacteriophage Charon 4A and a suitable E.coli host. Using this approach we have isolated and characterized several non-a globin clones from a Kurdish Jew with homozygous β thalassemia. The ability to isolate suitable amounts of high molecular weight DNA from peripheral blood provides a relatively simple means of constructing human gene libraries representing a variety of hemoglobin disorders.Keywords
This publication has 19 references indexed in Scilit:
- Complete nucleotide sequence of the human δ-globin geneCell, 1980
- The nucleotide sequence of the human β-globin geneCell, 1980
- Human fetal gγ- and Aγ-globin genes: Complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genesCell, 1980
- The primary structure of the human ε-globin geneCell, 1980
- The isolation of structural genes from libraries of eucaryotic DNACell, 1978
- Identification of a Nondeletion Defect in α-ThalassemiaNew England Journal of Medicine, 1977
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- A general method for isolation of high molecular weight DNA from eukaryotesNucleic Acids Research, 1976
- Agarose slab-gel electrophoresis equipmentAnalytical Biochemistry, 1975
- Isolation of High‐Molecular‐Weight DNA from Mammalian CellsJBIC Journal of Biological Inorganic Chemistry, 1973