Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen
- 1 January 2012
- journal article
- research article
- Published by CSIRO Publishing in Reproduction, Fertility and Development
- Vol. 24 (8), 1079-1083
- https://doi.org/10.1071/rd11088
Abstract
Much of the world’s food production is dependent on honey bees for pollination, and expanding food production will further increase the demand for managed pollination services. Apiculturists outside the native range of the honey bee, in the Americas, Australia and eastern Asia, have used only a few of the 27 described subspecies of honey bees (Apis mellifera) for beekeeping purposes. Within the endemic ranges of a particular subspecies, hybridisation can threaten native subspecies when local beekeepers import and propagate non-native honey bees. For many threatened species, cryopreserved germplasm can provide a resource for the preservation of diversity and recovery of endangered populations. However, although instrumental insemination of queen honey bees is well established, the absence of an effective means to cryopreserve honey bee semen has limited the success of efforts to preserve genetic diversity within the species or to develop repositories of honey bee germplasm for breeding purposes. Herein we report that some queens inseminated with cryopreserved semen were capable of producing a substantial number of fertilised offspring. These diploid female larvae were used to produce two additional sequential generations of new queens, which were then back-crossed to the same stock of frozen semen. Our results demonstrate the ability to produce queens using cryopreserved honey bee spermatozoa and the potential for the establishment of a honey bee genetic repository.Keywords
This publication has 19 references indexed in Scilit:
- A survey of honey bee colony losses in the United States, fall 2008 to spring 2009Journal of Apicultural Research, 2010
- Colony Collapse Disorder: A Descriptive StudyPLOS ONE, 2009
- Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratiosTheriogenology, 2009
- Gene flow in admixed populations and implications for the conservation of the Western honeybee, Apis melliferaJournal of Insect Conservation, 2008
- Genetic Diversity in Honey Bee Colonies Enhances Productivity and FitnessScience, 2007
- The Case of the Empty HivesScience, 2007
- New insights towards understanding the mechanisms of sperm protection by egg yolk and milkMolecular Reproduction and Development, 2006
- Genetic diversity within honeybee colonies prevents severe infections and promotes colony growthProceedings. Biological sciences, 2003
- Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvementAnimal Reproduction Science, 1995
- In vitro fertilization of oocytes by 37-year-old cryopreserved bovine spermatozoaTheriogenology, 1994