New insights towards understanding the mechanisms of sperm protection by egg yolk and milk
Top Cited Papers
- 26 July 2006
- journal article
- review article
- Published by Wiley in Molecular Reproduction and Development
- Vol. 73 (10), 1338-1344
- https://doi.org/10.1002/mrd.20565
Abstract
Mammalian sperm preservation in extenders containing egg yolk (EY) and/or milk has been used for over half a century. However, the mechanism by which EY or milk protects sperm during storage remains elusive. Studies conducted over the past two decades in our laboratory have revealed that a family of lipid‐binding proteins (BSP proteins) present in bull seminal plasma is detrimental to sperm preservation since these proteins induce cholesterol and phospholipid removal from the sperm membrane. Interestingly, these detrimental factors of seminal plasma interact with the low‐density lipoproteins (LDL) present in EY. This interaction minimizes lipid removal from the sperm membrane, which positively influences sperm storage in liquid or frozen states. Based on several lines of evidence, we suggest that the sequestration of BSP proteins by LDL (BSP proteins: lipoprotein interaction) is the major mechanism of sperm protection by EY. Skimmed milk, which is devoid of lipoproteins, also protects sperm during storage. Several studies indicate that the active components involved in sperm protection by milk are casein micelles. Thus, it appears that the mechanism by which milk protects sperm involves a BSP protein: casein micelle interaction. In view of these new insights, novel strategies have been suggested to improve the efficiency of semen preservation. Mol. Reprod. Dev.Keywords
This publication has 77 references indexed in Scilit:
- Identification of Proteins in the Accessory Sex Gland Fluid Associated With Fertility Indexes of Dairy Bulls: A Proteomic ApproachJournal of Andrology, 2006
- Isolation and characterization of the major proteins of ram seminal plasmaMolecular Reproduction and Development, 2005
- Isolation and characterization of glycosaminoglycans from bovine follicular fluid and their effect on sperm capacitationMolecular Reproduction and Development, 2005
- Isolation and Characterization of Gelatin-Binding Bison Seminal Vesicle Secretory ProteinsBiology of Reproduction, 2004
- Novel purification method for mammalian seminal plasma phospholipid‐binding proteins reveals the presence of a novel member of this family of protein in stallion seminal fluidMolecular Reproduction and Development, 2003
- Bovine Seminal Plasma Phospholipid-Binding Proteins Stimulate Phospholipid Efflux from Epididymal Sperm1Biology of Reproduction, 1999
- The roles of lipid and protein in the protection of ram spermatozoa at 5 C by egg-yolk lipoproteinReproduction, 1981
- The protection of ram and bull spermatozoa by the low density lipoprotein fraction of egg yolk during storage at 5°C and deep-freezingJournal of Thermal Biology, 1976