Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription
Open Access
- 12 December 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 1 (1), 189
- https://doi.org/10.1038/srep00189
Abstract
Cdc14 is an essential phosphatase in yeast but its role in the mammalian cell cycle remains obscure. We report here that Cdc14b-knockout cells display unscheduled induction of multiple cell cycle regulators resulting in early entry into DNA replication and mitosis from quiescence. Cdc14b dephosphorylates Ser5 at the C-terminal domain (CTD) of RNA polymerase II, a major substrate of cyclin-dependent kinases. Lack of Cdc14b results in increased CTD-Ser5 phosphorylation, epigenetic modifications that mark active chromatin, and transcriptional induction of cell cycle regulators. These data suggest a function for mammalian Cdc14 phosphatases in the control of transcription during the cell cycle.This publication has 52 references indexed in Scilit:
- Human Cdc14A Phosphatase Modulates the G2/M Transition through Cdc25A and Cdc25BOnline Journal of Public Health Informatics, 2010
- Vertebrate cells genetically deficient for Cdc14A or Cdc14B retain DNA damage checkpoint proficiency but are impaired in DNA repairThe Journal of cell biology, 2010
- Progression through the RNA Polymerase II CTD CycleMolecular Cell, 2009
- Modifications of RNA polymerase II are pivotal in regulating gene expression statesEMBO Reports, 2009
- PP1-mediated dephosphorylation of phosphoproteins at mitotic exit is controlled by inhibitor-1 and PP1 phosphorylationNature, 2009
- Cdk-counteracting phosphatases unlock mitotic exitCurrent Opinion in Cell Biology, 2008
- The Cdc14B-Cdh1-Plk1 Axis Controls the G2 DNA-Damage-Response CheckpointCell, 2008
- Genomic stability and tumour suppression by the APC/C cofactor Cdh1Nature, 2008
- BioGRID: a general repository for interaction datasetsNucleic Acids Research, 2006
- Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profilesProceedings of the National Academy of Sciences of the United States of America, 2005