Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay
- 1 January 2011
- journal article
- Published by International Research and Cooperation Association for Bio & Socio-Sciences Advancement (IRCA-BSSA) in Drug Discoveries & Therapeutics
- Vol. 5 (1), 18-25
- https://doi.org/10.5582/ddt.v5.1.18
Abstract
A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids with methyl ester residues. Treatment with β-glucanase attenuated the muscle contraction activity of the purified sample, suggesting that the β-glucan structure, probably as a branched form, was required for its activity. The purified fraction stimulated the production of interleukin-6 by mouse peritoneal macrophages. These results suggest that measuring immunostimulation in silkworm larvae is useful for evaluating innate immunostimulants from various sources.Keywords
This publication has 16 references indexed in Scilit:
- Porphyromonas gingivalis Peptidoglycans Induce Excessive Activation of the Innate Immune System in Silkworm Larvae*Journal of Biological Chemistry, 2010
- Insect Cytokine Paralytic Peptide (PP) Induces Cellular and Humoral Immune Responses in the Silkworm Bombyx moriPublished by Elsevier BV ,2010
- Targeting Receptor Tyrosine Kinases for Chemoprevention by Green Tea Catechin, EGCGInternational Journal of Molecular Sciences, 2008
- Activation of the Silkworm Cytokine by Bacterial and Fungal Cell Wall Components via a Reactive Oxygen Species-triggered MechanismPublished by Elsevier BV ,2008
- Beneficial Effects of Green Tea—A ReviewJournal of the American College of Nutrition, 2006
- Phase I study of green tea extract in patients with advanced lung cancerCancer Chemotherapy and Pharmacology, 2004
- Innate immune responses of a lepidopteran insect, Manduca sextaImmunological Reviews, 2004
- Effects of silkworm paralytic peptide on in vitro hematopoiesis and plasmatocyte spreadingArchives of Insect Biochemistry and Physiology, 2003
- Phylogenetic Perspectives in Innate ImmunityScience, 1999
- Bactericidal substance induced in the haemolymph of Sarcophaga peregrina larvaeJournal of Insect Physiology, 1977