Bixin regulates mRNA expression involved in adipogenesis and enhances insulin sensitivity in 3T3-L1 adipocytes through PPARγ activation
- 3 November 2009
- journal article
- research article
- Published by Elsevier BV in Biochemical and Biophysical Research Communications
- Vol. 390 (4), 1372-1376
- https://doi.org/10.1016/j.bbrc.2009.10.162
Abstract
Insulin resistance is partly due to suppression of insulin-induced glucose uptake into adipocytes. The uptake is dependent on adipocyte differentiation, which is controlled at mRNA transcription level. The peroxisome proliferator-activated receptor (PPAR), a ligand-regulated nuclear receptor, is involved in the differentiation. Many food-derived compounds serve as ligands to activate or inactivate PPAR. In this study, we demonstrated that bixin and norbixin (annatto extracts) activate PPARγ by luciferase reporter assay using GAL4–PPAR chimera proteins. To examine the effects of bixin on adipocytes, 3T3-L1 adipocytes were treated with bixin or norbixin. The treatment induced mRNA expression of PPARγ target genes such as adipocyte-specific fatty acid-binding protein (aP2), lipoprotein lipase (LPL), and adiponectin in differentiated 3T3-L1 adipocytes and enhanced insulin-dependent glucose uptake. The observations indicate that bixin acts as an agonist of PPARγ and enhances insulin sensitivity in 3T3-L1 adipocytes, suggesting that bixin is a valuable food-derived compound as a PPAR ligand to regulate lipid metabolism and to ameliorate metabolic syndrome.Funding Information
- Ministry of Education, Culture, Sports, Science and Technology (15081205, 19380074, 19780096)
This publication has 23 references indexed in Scilit:
- PPAR and human metabolic diseaseJCI Insight, 2006
- Relative Inhibition of Lipid Peroxidation, Cyclooxygenase Enzymes, and Human Tumor Cell Proliferation by Natural Food ColorsJournal of Agricultural and Food Chemistry, 2005
- DNA damage and aberrant crypt foci as putative biomarkers to evaluate the chemopreventive effect of annatto (Bixa orellana L.) in rat colon carcinogenesisMutation Research - Genetic Toxicology and Environmental Mutagenesis, 2005
- PPARs and the complex journey to obesityNature Medicine, 2004
- Dietary supplementation with a natural carotenoid mixture decreases oxidative stressEuropean Journal of Clinical Nutrition, 2003
- Norbixin ingestion did not induce any detectable DNA breakage in liver and kidney but caused a considerable impairment in plasma glucose levels of rats and miceThe Journal of Nutritional Biochemistry, 2002
- Antioxidant action of bixin against cisplatin-induced chromosome aberrations and lipid peroxidation in ratsPharmacological Research, 2001
- PPARγ Is Required for Placental, Cardiac, and Adipose Tissue DevelopmentMolecular Cell, 1999
- PPARγ Is Required for the Differentiation of Adipose Tissue In Vivo and In VitroMolecular Cell, 1999
- PPARγ Mediates High-Fat Diet–Induced Adipocyte Hypertrophy and Insulin ResistanceMolecular Cell, 1999