Aspirin commits yeast cells to apoptosis depending on carbon source
Open Access
- 1 January 2004
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 150 (1), 109-115
- https://doi.org/10.1099/mic.0.26578-0
Abstract
The effect of aspirin on the growth of a wild-type Saccharomyces cerevisiae strain (EG103), containing both copper,zinc superoxide dismutase (CuZnSOD) and manganese superoxide dismutase (MnSOD), a strain deficient in MnSOD (EG110) and a strain deficient in CuZnSOD (EG118) was measured in media containing different carbon sources. Aspirin inhibited the fermentative growth of all three strains in glucose medium. It inhibited the non-fermentative growth of the MnSOD-deficient strain very drastically in ethanol medium and had no effect on this strain in glycerol or acetate medium. The non-fermentative growth of the other two strains was not affected by aspirin. The growth inhibition of strain EG110 was associated with early necrosis in glucose medium and late apoptosis in ethanol medium. The apoptosis was preceded by a pronounced loss of cell viability. The growth inhibitory effect of aspirin was not reversed by the antioxidants N-acetylcysteine and vitamin E. Furthermore, aspirin itself appeared to act as an antioxidant until the onset of overt apoptosis, when a moderate increase in the intracellular oxidation level occurred. This suggested that reactive oxygen species probably do not play a primary role in the apoptosis of cells exposed to aspirin.Keywords
This publication has 17 references indexed in Scilit:
- CytochromecRelease and Mitochondria Involvement in Programmed Cell Death Induced by Acetic Acid inSaccharomyces cerevisiaeMolecular Biology of the Cell, 2002
- A Caspase-Related Protease Regulates Apoptosis in YeastMolecular Cell, 2002
- Aspirin induces cell death and caspase-dependent phosphatidylserine externalization in HT-29 human colon adenocarcinoma cellsBritish Journal of Cancer, 1999
- Oxygen Stress: A Regulator of Apoptosis in YeastThe Journal of cell biology, 1999
- Mammalian Bax triggers apoptotic changes in yeastFEBS Letters, 1998
- Superoxide in ApoptosisPublished by Elsevier BV ,1998
- A Yeast Mutant Showing Diagnostic Markers of Early and Late ApoptosisThe Journal of cell biology, 1997
- Mitochondrial superoxide dismutase is essential for ethanol tolerance of Saccharomyces cerevisiae in the post-diauxic phaseMicrobiology, 1997
- Intracellular Adenosine Triphosphate (ATP) Concentration: A Switch in the Decision Between Apoptosis and NecrosisThe Journal of Experimental Medicine, 1997
- Superoxide Dismutase Activity Is Essential for Stationary Phase Survival in Saccharomyces cerevisiae: MITOCHONDRIAL PRODUCTION OF TOXIC OXYGEN SPECIES IN VIVOJournal of Biological Chemistry, 1996