Isolation of a candidate human hematopoietic stem-cell population.
- 1 April 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 89 (7), 2804-2808
- https://doi.org/10.1073/pnas.89.7.2804
Abstract
We have identified a rare (0.05-0.1%) subset of human fetal bone marrow cells that contains multipotent hematopoietic precursors. The population of human precursor cells that express Thy-1 and CD34 but no known lineage markers is enriched for clonogenic activity that establishes long-term, multilineage (myelomonocytic and B lymphoid) cultures on mouse marrow stromal lines. Further, the Thy-1+CD34+ subset that takes up little of the fluorescent mitochondrial dye rhodamine 123 contains virtually all the cells that establish long-term cultures. In human fetal thymus transplanted into SCID (severe combined immunodeficiency) mice, Thy-1+CD34+ fetal bone marrow cells differentiate into T lymphocytes. In two of nine cases, allogeneic Thy-1+CD34+ cells could engraft intact human fetal bone marrow grown in SCID mice, resulting in donor-derived myeloid and B cells. By extrapolation, the rare human Thy-1+Lin-CD34+ cell population contains pluripotent hematopoietic progenitors; we propose that it is highly enriched for candidate hematopoietic stem cells.Keywords
This publication has 44 references indexed in Scilit:
- Preclinical Evaluation of Human Hematolymphoid Function in the SCID‐hu MouseImmunological Reviews, 1991
- Lymphoid reconstitution of the human fetal thymus in SCID mice with CD34+ precursor cells.The Journal of Experimental Medicine, 1991
- Identification of a subset of murine natural killer cells that mediates rejection of Hh-1d but not Hh-1b bone marrow grafts.The Journal of Experimental Medicine, 1989
- Parallel processing data acquisition system for multilaser flow cytometry and cell sortingCytometry, 1989
- Antigen CD34+ marrow cells engraft lethally irradiated baboons.JCI Insight, 1988
- Bone marrow stromal cell lines with lymphopoietic activity express high levels of a pre-B neoplasia-associated moleculeCell, 1987
- Isolation of two early B lymphocyte progenitors from mouse marrow: A committed Pre-Pre-B cell and a clonogenic Thy-1lo hematopoietic stem cellCell, 1986
- Studies of thymocytopoiesis in rats and mice. I. Kinetics of appearance of thymocytes using a direct intrathymic adoptive transfer assay for thymocyte precursors.The Journal of Experimental Medicine, 1986
- Bone marrow cells give rise to distinct cell clones within the thymusNature, 1984
- Adherence column and buoyant density separation of bone marrow stem cells and more differentiated cellsJournal of Cellular Physiology, 1971