Antigen CD34+ marrow cells engraft lethally irradiated baboons.
Open Access
- 1 March 1988
- journal article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 81 (3), 951-955
- https://doi.org/10.1172/jci113409
Abstract
The CD34 antigen is present on 1-4% of human marrow cells including virtually all hematopoietic progenitors detected by in vitro assays. Since the anti-CD34 monoclonal antibody 12-8 reacts with a similar marrow population in baboons, it was possible to test whether this antigen is expressed by stem cells responsible for hematopoietic reconstitution in vivo. CD34+ cells were enriched from marrows of five baboons using avidin-biotin immunoadsorption. After lethal irradiation, the five animals were given 15-27 X 10(6) autologous marrow cells (3.2-4.4 X 10(6) cells/kg) containing 65-91% CD34+ cells. All animals achieved granulocyte counts greater than 1,000/mm3 and platelet counts greater than 20 X 10(3)/mm3 by 13-24 d posttransplant and subsequently developed normal peripheral blood counts. Two additional animals received 184 and 285 X 10(6) marrow cells/kg depleted of CD34+ cells. One animal died at day 29 without engraftment, while the other had pancytopenia for greater than 100 d posttransplant. The data suggest that stem cells responsible for hematopoietic reconstitution are CD34+.Keywords
This publication has 15 references indexed in Scilit:
- Recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) shortens the period of neutropenia after autologous bone marrow transplantation in a primate model.JCI Insight, 1987
- Engraftment of dogs with Ia-positive marrow cells isolated by avidin-biotin immunoadsorption.1987
- The L4F3 antigen is expressed by unipotent and multipotent colony-forming cells but not by their precursors.1986
- Positive selection of viable cell populations using avidin-biotin immunoadsorptionJournal of Immunological Methods, 1986
- MONOCLONAL-ANTIBODY 12-8 RECOGNIZES A 115-KD MOLECULE PRESENT ON BOTH UNIPOTENT AND MULTIPOTENT HEMATOPOIETIC COLONY-FORMING CELLS AND THEIR PRECURSORS1986
- Elimination of Daudi lymphoblasts from human bone marrow using avidin-biotin immunoadsorption.1986
- A novel monoclonal antibody BI-3C5 recognises myeloblasts and non-B non-T lymphoblasts in acute leukaemias and CGL blast crises, and reacts with immature cells in normal bone marrowLeukemia Research, 1985
- Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.The Journal of Immunology, 1984
- Demonstration of hemopoietic stem cells in the peripheral blood of baboons by cross circulation.1977
- Low temperature preservation of monkey marrow in dimethyl sulfoxideCryobiology, 1970