First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhood
Open Access
- 16 September 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 13 (1), 433
- https://doi.org/10.1186/1471-2334-13-433
Abstract
Background: Non-severe acute respiratory syndrome (non-SARS)-related human coronaviruses (HCoVs), including HCoV-229E, -HKU1, -NL63, and -OC43, have been detected in respiratory tract samples from children and adults. However, the natural prevalence of antibodies against these viruses in serum among population is unknown. Methods: To measure antibodies to the spike (S) protein of the four common non-SARS HCoVs, recombinant S proteins of the four HCoVs were expressed and characterised in 293 T cell. An S-protein-based indirect immunofluorescence assay (IFA) was then developed to detect anti-S IgG and IgM for the four individual HCoVs and applied to serum samples from a general asymptomatic population (218 children and 576 adults) in Beijing. Results: Of 794 blood samples tested, only 29 (3.65%) were negative for anti-S IgG. The seropositivity of the four anti-S IgG antibodies was >70% within the general population. The majority of seroconversions to four-HCoV positivity first occurred in children. Both S-IgG and S-IgM antibodies were detectable among children and increased with age, reaching a plateau at 6 years of age. However, no anti-S IgM was detected in healthy adults. Conclusion: Large proportions of children and adults in Beijing have evidence of anti-S IgG against four the HCoVs, and first infections by all four non-SARS HCoVs takes place during childhood.This publication has 33 references indexed in Scilit:
- Update on Rhinovirus and Coronavirus InfectionsSeminars in Respiratory and Critical Care Medicine, 2011
- Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virusJournal of Clinical Virology, 2009
- A line immunoassay utilizing recombinant nucleocapsid proteins for detection of antibodies to human coronavirusesDiagnostic Microbiology and Infectious Disease, 2008
- Seroepidemiology of group I human coronaviruses in childrenJournal of Clinical Virology, 2007
- Recombinant Protein-Based Assays for Detection of Antibodies to Severe Acute Respiratory Syndrome Coronavirus Spike and Nucleocapsid ProteinsClinical and Vaccine Immunology, 2007
- A DNA vaccine induces SARS coronavirus neutralization and protective immunity in miceNature, 2004
- Identification of a new human coronavirusNature Medicine, 2004
- The time course of the immune response to experimental coronavirus infection of manEpidemiology and Infection, 1990
- Prevalence of human coronavirus antibody in the population of southern iraqJournal of Medical Virology, 1982
- Coronavirus antibody titres in sera of healthy adults and experimentally infected volunteersEpidemiology and Infection, 1972