Identification of a new human coronavirus
Top Cited Papers
- 21 March 2004
- journal article
- Published by Springer Science and Business Media LLC in Nature Medicine
- Vol. 10 (4), 368-373
- https://doi.org/10.1038/nm1024
Abstract
Three human coronaviruses are known to exist: human coronavirus 229E (HCoV-229E), HCoV-OC43 and severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV). Here we report the identification of a fourth human coronavirus, HCoV-NL63, using a new method of virus discovery. The virus was isolated from a 7-month-old child suffering from bronchiolitis and conjunctivitis. The complete genome sequence indicates that this virus is not a recombinant, but rather a new group 1 coronavirus. The in vitro host cell range of HCoV-NL63 is notable because it replicates on tertiary monkey kidney cells and the monkey kidney LLC-MK2 cell line. The viral genome contains distinctive features, including a unique N-terminal fragment within the spike protein. Screening of clinical specimens from individuals suffering from respiratory illness identified seven additional HCoV-NL63-infected individuals, indicating that the virus was widely spread within the human populatioKeywords
This publication has 34 references indexed in Scilit:
- Coronavirus 229E-Related Pneumonia in Immunocompromised PatientsClinical Infectious Diseases, 2003
- Unique and Conserved Features of Genome and Proteome of SARS-coronavirus, an Early Split-off From the Coronavirus Group 2 LineageJournal of Molecular Biology, 2003
- The Genome Sequence of the SARS-Associated CoronavirusScience, 2003
- Characterization of a Novel Coronavirus Associated with Severe Acute Respiratory SyndromeScience, 2003
- SARS Virus: The Beginning of the Unraveling of a New CoronavirusJournal of Biomedical Science, 2003
- Viral Induced DemyelinationBrain Pathology, 2001
- Coronavirus Infection in Acute Lower Respiratory Tract Disease of InfantsThe Journal of Infectious Diseases, 1974
- Effects of a "new" human respiratory virus in volunteers.BMJ, 1967
- A New Virus Isolated from the Human Respiratory Tract.Experimental Biology and Medicine, 1966
- Cultivation of a Novel Type of Common-cold Virus in Organ CulturesBMJ, 1965