Deletion of a DNA Polymerase β Gene Segment in T Cells Using Cell Type-Specific Gene Targeting
- 1 July 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 265 (5168), 103-106
- https://doi.org/10.1126/science.8016642
Abstract
Deletion of the promoter and the first exon of the DNA polymerase beta gene (pol beta) in the mouse germ line results in a lethal phenotype. With the use of the bacteriophage-derived, site-specific recombinase Cre in a transgenic approach, the same mutation can be selectively introduced into a particular cellular compartment-in this case, T cells. The impact of the mutation on those cells can then be analyzed because the mutant animals are viable.This publication has 12 references indexed in Scilit:
- Independent control of immunoglobulin switch recombination at individual switch regions evidenced through Cre-loxP-mediated gene targetingCell, 1993
- Tissue- and site-specific DNA recombination in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Targeted oncogene activation by site-specific recombination in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Generation and Analysis of Interleukin-4 Deficient MiceScience, 1991
- Stable Expression and Somatic Hypermutation of Antibody V Regions in B-Cell Developmental PathwaysAnnual Review of Immunology, 1989
- Difference in the expression level of DNA polymerase β among mouse tissues: High expression in the pachytene spermatocyteExperimental Cell Research, 1989
- Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.Proceedings of the National Academy of Sciences of the United States of America, 1988
- Development of the Primary Antibody RepertoireScience, 1987
- Bacteriophage P1 site-specific recombination: I. Recombination between loxP sitesJournal of Molecular Biology, 1981
- Origin of Antibody VariationNature, 1966