Bacterial Endosymbiosis Is Widely Present among Zygomycetes but Does Not Contribute to the Pathogenesis of Mucormycosis
Open Access
- 1 October 2008
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 198 (7), 1083-1090
- https://doi.org/10.1086/591461
Abstract
Environmental isolates of the fungus Rhizopus have been shown to harbor a bacterial endosymbiont (Burkholderia) that produces rhixozin, a plant mycotoxin. Wesought to define the role of rhizoxin production by endosymbionts in the pathogenesis of mucormycosis. Endosymbiotic bacteria were identified by polymerase chain reaction in 15 (54%) of 28 clinical isolates of Zygomycetes, with 33% of the bacterial strains showing ⩾87% identity to Burkholderia 16S rDNA. The presence of rhizoxin in myclial extracts from fungi harboring bacteria was confirmed by high-performance liquid chromatography analysis. However, fungal strains with or without endosymbionts did not differ in their ability to cause endothelial cell injury in vitro, nor did antibiotic-mediated eradication of endosymbionts and rhizoxin production decrease the virulence of fungal strains in mice or flies. In summary, although bacterial endosymbiosis is widely detected in clinical isolates of Zygomycetes, including Rhizopus oryzae strains, we found no evidence that bacterial endosymbionts and rhizoxin contribute to the pathogenesis of mucormycosis in the models studied.Keywords
This publication has 27 references indexed in Scilit:
- Improved Outcome of Zygomycosis in Patients with Hematological Diseases?Leukemia & Lymphoma, 2004
- Iteration as Programmed Event during Polyketide Assembly; Molecular Analysis of the Aureothin Biosynthesis Gene ClusterCell Chemical Biology, 2003
- Liposomal Amphotericin B, and Not Amphotericin B Deoxycholate, Improves Survival of Diabetic Mice Infected with Rhizopus oryzaeAntimicrobial Agents and Chemotherapy, 2003
- Opportunistic Mycelial Fungal Infections in Organ Transplant Recipients: Emerging Importance of Non-Aspergillus Mycelial FungiClinical Infectious Diseases, 2003
- Phase II clinical trials with rhizoxin in breast cancer and melanomaBritish Journal of Cancer, 1996
- Prelinical and Phase I Studies With Rhizoxin to Apply a Pharmacokinetically Guided Dose-Escalation SchemeJNCI Journal of the National Cancer Institute, 1992
- Studies on macrocyclic lactone antibiotics. XI. Anti-mitotic and anti-tubulin activity of new antitumor antibiotics, rhizoxin and its homologues.The Journal of Antibiotics, 1987
- Rhizoxin, a macrocyclic lactone antibiotic, as a new antitumor agent against human and murine tumor cells and their vincristine-resistant sublines.1986
- Studies on macrocyclic lactone antibiotics. VIII. Absolute structures of rhizoxin and a related compound.The Journal of Antibiotics, 1986
- Culture of Human Endothelial Cells Derived from Umbilical Veins. IDENTIFICATION BY MORPHOLOGIC AND IMMUNOLOGIC CRITERIAJCI Insight, 1973