CpG island methylation in aberrant crypt foci and cancers from the same patients
- 11 May 2005
- journal article
- Published by Wiley in International Journal of Cancer
- Vol. 115 (5), 747-751
- https://doi.org/10.1002/ijc.20936
Abstract
Aberrant methylation of CpG islands is a common alteration in human colon cancer. Methylation of only a limited number of loci has been studied in aberrant crypt foci (ACF), the earliest identified premalignant lesions in the colon, and in only a limited number of lesions from the same patients. Methylation‐specific PCR was used to analyze 35 ACF, samples of normal crypts with the same number of crypts as were in each ACF and 22 cancers from the same patients. Aberrant methylation in cellular retinol‐binding protein 1 (CRBP1), MINT31 or H‐cadherin (CDH13) was identified in 19 of 35 (54%) ACF. Hypermethylation of CRBP1, MINT31 or CDH13 was more frequent (15 of 22, 68%) in cancers. DNA methylation of CRBP1, MINT31 or CDH13 was not correlated between cancers and ACF from the same patients. Aberrant methylation was not correlated with patient age, number of crypts in the ACF or cancer stage. These results suggest that hypermethylation in the promoter region of some genes is an independent event, occurs early in human colon tumorigenesis and may play an important role during the transformation and progression of some lesions to colon cancers.Keywords
This publication has 14 references indexed in Scilit:
- Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancerNature Genetics, 2004
- Gene Silencing in Cancer in Association with Promoter HypermethylationNew England Journal of Medicine, 2003
- SLC5A8 , a sodium transporter, is a tumor suppressor gene silenced by methylation in human colon aberrant crypt foci and cancersProceedings of the National Academy of Sciences of the United States of America, 2003
- CpG Island Methylation in Aberrant Crypt Foci of the ColorectumThe American Journal of Pathology, 2002
- Concordant CpG Island Methylation in Hyperplastic PolyposisThe American Journal of Pathology, 2002
- CpG Island Methylation in Colorectal AdenomasThe American Journal of Pathology, 2001
- Analysis of K-ras, APC, and β-catenin in aberrant crypt foci in sporadic adenoma, cancer, and familial adenomatous polyposisGastroenterology, 2001
- Distinct genetic profiles in colorectal tumors with or without the CpG island methylator phenotypeProceedings of the National Academy of Sciences of the United States of America, 2000
- CpG island methylator phenotype in colorectal cancerProceedings of the National Academy of Sciences of the United States of America, 1999
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences of the United States of America, 1996