In-Fusion™ Assembly: Seamless Engineering of Multidomain Fusion Proteins, Modular Vectors, and Mutations
Open Access
- 1 September 2007
- journal article
- technical report
- Published by Taylor & Francis Ltd in BioTechniques
- Vol. 43 (3), 354-359
- https://doi.org/10.2144/000112536
Abstract
In-Fusion™ can join any two pieces of DNA that have a 15-bp overlap at their ends. The result is equivalent to a recombination event at the ends of the DNAs. The 15-bp overlap may be engineered by inclusion in primers used to...Funding Information
- National Institutes of Health (AI39671, AI54456, and AI56299)
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