Selective Activation of Transcription by a Novel CCAAT Binding Factor
- 29 July 1988
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 241 (4865), 582-585
- https://doi.org/10.1126/science.3399893
Abstract
A novel CCAAT binding factor (CBF) composed of two different subunits has been extensively purified from rat liver. Both subunits are needed for specific binding to DNA. Addition of this purified protein to nuclear extracts of NIH 3T3 fibroblasts stimulates transcription from several promoters including the alpha 2(I) collagen, the alpha 1(I) collagen, the Rous sarcoma virus long terminal repeat (RSV-LTR), and the adenovirus major late promoter. Point mutations in the CCAAT motif that show either no binding or a decreased binding of CBF likewise abolish or reduce activation of transcription by CBF. Activation of transcription requires, therefore, the specific binding of CBF to its recognition sites.Keywords
This publication has 22 references indexed in Scilit:
- A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeableCell, 1988
- Human CCAAT-binding proteins have heterologous subunitsCell, 1988
- A multiplicity of CCAAT box-binding proteinsCell, 1987
- Interactions between a DNA-binding transcription factor (COUP) and a non-DNA binding factor (S300-II)Cell, 1987
- Regulation of Inducible and Tissue-Specific Gene ExpressionScience, 1987
- Deletion analysis of GAL4 defines two transcriptional activating segmentsCell, 1987
- A cellular DNA-binding protein that activates eukaryotic transcription and DNA replicationCell, 1987
- Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of YeastCell, 1986
- Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box regionCell, 1985
- Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteinsNature, 1985