A multiplicity of CCAAT box-binding proteins
- 1 September 1987
- journal article
- research article
- Published by Elsevier BV in Cell
- Vol. 50 (6), 863-872
- https://doi.org/10.1016/0092-8674(87)90513-7
Abstract
NF-Y is a sequence-specific DNA-binding protein that recognizes the Y box, a promoter element common to all major histocompatibility complex class II genes. Since the 14-base Y element harbors a CCAAT box in reverse, we were prompted to ask whether NF-Y is actually a CCAAT box-binding protein and whether it is related to the previously described CCAAT-binding factors CBP and CTF/NF-I. Data from gel retardation, methylation interference, saturation mutagenesis, and cross-competition experiments establish definitively that NF-Y is an entirely distinct CCAAT box-binding entity. Moreover, these experiments have uncovered a fourth CCAAT-binding protein, NF-Y∗, that interacts with the thymidine kinase promoter. Clearly, then, there exists a multiplicity of factors that recognize CCAAT sequences; it now becomes imperative to understand the functional significance of this multiplicity.Keywords
This publication has 45 references indexed in Scilit:
- A Genetic Approach to Promoter Recognition During trans Induction of Viral Gene ExpressionScience, 1986
- Heat shock regulatory elements function as an inducible enhancer in the xenopus hsp70 gene and when linked to a heterologous promoterCell, 1986
- Partial purification of a nuclear protein that binds to the CCAAT box of the mouse alpha 1-globin gene.Molecular and Cellular Biology, 1986
- Molecular Genetics of the Human Major Histocompatibility ComplexPublished by Springer Science and Business Media LLC ,1986
- An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoterCell, 1985
- A phage repressor–operator complex at 7 Å resolutionNature, 1985
- Linker scanning mutagenesis of the 5'-flanking region of the mouse beta-major-globin gene: sequence requirements for transcription in erythroid and nonerythroid cells.Molecular and Cellular Biology, 1985
- A point mutation in the Aγ-globin gene promoter in Greek hereditary persistence of fetal haemoglobinNature, 1985
- ROLE OF ABERRANT HLA-DR EXPRESSION AND ANTIGEN PRESENTATION IN INDUCTION OF ENDOCRINE AUTOIMMUNITYThe Lancet, 1983
- Mechanics of sequence-dependent stacking of bases in B-DNAJournal of Molecular Biology, 1982