Wavefront correction in two-photon microscopy with a multi-actuator adaptive lens
Open Access
- 21 May 2018
- journal article
- research article
- Published by Optica Publishing Group in Optics Express
- Vol. 26 (11), 14278-14287
- https://doi.org/10.1364/oe.26.014278
Abstract
A multi-actuator adaptive lens (AL) was incorporated into a multi-photon (MP) microscope to improve the quality of images of thick samples. Through a hill-climbing procedure the AL corrected for the specimen-induced aberrations enhancing MP images. The final images hardly differed when two different metrics were used, although the sets of Zernike coefficients were not identical. The optimized MP images acquired with the AL were also compared with those obtained with a liquid-crystal-on-silicon spatial light modulator. Results have shown that both devices lead to similar images, which corroborates the usefulness of this AL for MP imaging.Keywords
Funding Information
- Fundación Séneca (f SéNeCa) (19897/GERM/15)
- H2020 European Research Council (ERC) (ERC-2013-AdG-339228)
- Secretaría de Estado de Investigación, Desarrollo e Innovación (SEIDI) (FIS2016-76163-R)
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