Adaptive correction of depth‐induced aberrations in multiphoton scanning microscopy using a deformable mirror
- 1 April 2002
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 206 (1), 65-71
- https://doi.org/10.1046/j.1365-2818.2002.01004.x
Abstract
We demonstrate adaptive aberration correction for depth‐induced spherical aberration in a multiphoton scanning microscope with a micromachined deformable mirror. Correction was made using a genetic learning algorithm with two‐photon fluorescence intensity feedback to determine the desired shape for an adaptive mirror. For a 40×/0.6 NA long working distance objective, the axial scanning range was increased from 150 mm to 600 mm.Keywords
This publication has 15 references indexed in Scilit:
- Adaptive aberration correction in a two‐photon microscopeJournal of Microscopy, 2000
- Aberration correction for confocal imaging in refractive‐index‐mismatched mediaJournal of Microscopy, 1998
- The role of specimen‐induced spherical aberration in confocal microscopyJournal of Microscopy, 1997
- Technology and applications of micromachined silicon adaptive mirrorsOptical Engineering, 1997
- Achromatic three-wave (or more) lateral shearing interferometerJournal of the Optical Society of America A, 1995
- Optical third-harmonic generation at interfacesPhysical Review A, 1995
- Electromagnetic diffraction of light focused through a planar interface between materials of mismatched refractive indices: an integral representationJournal of the Optical Society of America A, 1995
- Aberrations in confocal fluorescence microscopy induced by mismatches in refractive indexJournal of Microscopy, 1993
- Aberration compensation in confocal microscopyApplied Optics, 1991
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990