Impact of Sampling Parameters on the Radical Scavenging Potential of Olive (Olea europaea L.) Leaves
- 31 March 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 57 (9), 3470-3477
- https://doi.org/10.1021/jf900171d
Abstract
The impact of sampling parameters, that is, cultivar, leaf age, and sampling date, on the radical scavenging potential of olive leaf extracts was examined via the DPPH• and other assays. Total phenol content was estimated colorimetrically and by fluorometry, whereas phenol composition was assessed by RP-HPLC coupled with diode array, fluorometric, and MS detection systems. Oleuropein was not always the major leaf constituent. Considerable differences noted in individual phenol levels (hydroxytyrosol, oleuropein and other secoiridoids, verbascoside, and flavonoids) among samples were not reflected either in the total phenol content or in the radical scavenging potential of the extracts. It can be suggested that olive leaf is a robust source of radical scavengers throughout the year and that differentiation in the levels of individual components depends rather on sampling period than on cultivar or age. The latter does not present predictable regularity. Exploitation of all types of leaves expected in an olive tree shoot for the extraction of bioactive compounds is feasible.This publication has 26 references indexed in Scilit:
- Ultrasound-assisted extraction and silylation prior to gas chromatography–mass spectrometry for the characterization of the triterpenic fraction in olive leavesJournal of Chromatography A, 2007
- Polyphenolic content and in vitro antioxidant characteristics of wine industry and other agri-food solid waste extractsJournal of Food Composition and Analysis, 2007
- Changes in oleuropein levels during differentiation and development of floral buds in ‘Arbequina’ olivesScientia Horticulturae, 2006
- Factors Affecting the Contents of Iridoid Oleuropein in Olive Leaves (Olea europaea L.)Journal of Agricultural and Food Chemistry, 2005
- Countercurrent Supercritical Fluid Extraction and Fractionation of High-Added-Value Compounds from a Hexane Extract of Olive LeavesJournal of Agricultural and Food Chemistry, 2004
- Quantitative Changes in Phenolic Content during Physiological Development of the Olive (Olea europaea) Cultivar Hardy's MammothJournal of Agricultural and Food Chemistry, 2003
- Rapid High-Performance Liquid Chromatography Analysis for the Quantitative Determination of Oleuropein in Olea europaea LeavesJournal of Agricultural and Food Chemistry, 2001
- Oleuropein and related compoundsJournal of the Science of Food and Agriculture, 2000
- Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profileJournal of the Science of Food and Agriculture, 2000
- Antioxidant activity of phenolics extracted from Olea europaea L. leavesFood Chemistry, 2000