Factors Affecting the Contents of Iridoid Oleuropein in Olive Leaves (Olea europaea L.)
- 17 December 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (2), 434-440
- https://doi.org/10.1021/jf051647b
Abstract
In this study, for the first time, the impact of the genetic factor on the contents of oleuropein in olive leaves was not only evaluated but the influence exerted by the color/age of leaves (green, green-yellowish, and yellow) and the collecting period (spring or autumn) was also evaluated. A repetitive high-resolution gas chromatographic quantitation method and an accurate high-performance liquid chromatographic method were developed. These analytical methods gave results showing a highly linear relationship. Samples of olive leaves were taken from seven major Italian olive cultivars, such as Dritta, Leccino, Caroleo, Coratina, Castiglionese, Nebbio, and Grossa di Cassano. Such a vegetal raw material could actually be exploited for recovering oleuropein, considered to be a high-added value molecule. This could be converted into hydrxytyrosol, a compound known to possess strong bioactive properties. Olive leaves showed considerable contents of oleuropein, which with some cultivars were even higher with respect to those present in the corresponding olive fruits (reported in the literature). The amounts of oleuropein in the collected leaves were markedly modified by the color/age and genetic factors, whereas meaningless variations were ascribable to the quantitation method and the collecting period factors. Various chemometrics, applied to the obtained analytical data, appeared to be effective in discriminating the samples on the basis of the above-examined experimental factors, thus confirming how these should be taken into account in future industrial recovery of oleuropein from olive leaves.This publication has 12 references indexed in Scilit:
- Bioactive constituents, flavors and aromas of virgin oils obtained by processing olives with a natural enzyme extractEuropean Journal of Lipid Science and Technology, 2004
- Supplementation of oils with oleanolic acid from the olive leaf (olea europaea)European Journal of Lipid Science and Technology, 2004
- Olea europaeaL. Leaf Extract and Derivatives: Antioxidant PropertiesJournal of Agricultural and Food Chemistry, 2002
- Changes in phenolic and enzymatic activities content during fruit ripening in two Italian cultivars of Olea europaea L.Plant Science, 2002
- Bioactive derivatives from oleuropein by a biotransformation on Olea europaea leaf extractsJournal of Biotechnology, 2001
- Antioxidant Activity of the Main Bioactive Derivatives from Oleuropein Hydrolysis by Hyperthermophilic β-GlycosidaseJournal of Agricultural and Food Chemistry, 2001
- Hydroxytyrosol, as a component of olive mill waste water, is dose- dependently absorbed and increases the antioxidant capacity of rat plasmaFree Radical Research, 2001
- Antioxidant activity of phenolics extracted from Olea europaea L. leavesFood Chemistry, 2000
- Hydrolysis of oleuropein by recombinant β-glycosidase from hyperthermophilic archaeon Sulfolobus solfataricus immobilised on chitosan matrixJournal of Biotechnology, 2000
- Expression and extensive characterization of a β-glycosidase from the extreme thermoacidophilic archaeon Sulfolobus solfataricus in Escherichia coli: Authenticity of the recombinant enzymeEnzyme and Microbial Technology, 1995