Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray
Top Cited Papers
Preprint
- 25 March 2020
- preprint
- Published by Cold Spring Harbor Laboratory
Abstract
The current practice for diagnosis of SARS-CoV-2 infection relies on PCR testing of nasopharyngeal or respiratory specimens in a symptomatic patient at high epidemiologic risk. This testing strategy likely underestimates the true prevalence of infection, creating the need for serologic methods to detect infections missed by the limited testing to date. Here, we describe the development of a coronavirus antigen microarray containing immunologically significant antigens from SARS-CoV-2, in addition to SARS-CoV, MERS-CoV, common human coronavirus strains, and other common respiratory viruses. A preliminary study of human sera collected prior to the SARS-CoV-2 pandemic demonstrates overall high IgG reactivity to common human coronaviruses and low IgG reactivity to epidemic coronaviruses including SARS-CoV-2, with some cross-reactivity of conserved antigenic domains including S2 domain of spike protein and nucleocapsid protein. This array can be used to answer outstanding questions regarding SARS-CoV-2 infection, including whether baseline serology for other coronaviruses impacts disease course, how the antibody response to infection develops over time, and what antigens would be optimal for vaccine development.Keywords
This publication has 10 references indexed in Scilit:
- Ventilation and laboratory confirmed acute respiratory infection (ARI) rates in college residence halls in College Park, MarylandEnvironment International, 2020
- Use of an Influenza Antigen Microarray to Measure the Breadth of Serum Antibodies Across Virus SubtypesJournal of Visualized Experiments, 2019
- Protein Microarray Analysis of the Specificity and Cross-Reactivity of Influenza Virus Hemagglutinin-Specific AntibodiesmSphere, 2018
- Evaluation of quantum dot immunofluorescence and a digital CMOS imaging system as an alternative to conventional organic fluorescence dyes and laser scanning for quantifying protein microarraysProteomics, 2016
- Review ArticleEpidemiology, 2015
- Evaluation of Serologic and Antigenic Relationships Between Middle Eastern Respiratory Syndrome Coronavirus and Other Coronaviruses to Develop Vaccine Platforms for the Rapid Response to Emerging CoronavirusesThe Journal of Infectious Diseases, 2013
- First infection by all four non-severe acute respiratory syndrome human coronaviruses takes place during childhoodBMC Infectious Diseases, 2013
- Specific serology for emerging human coronaviruses by protein microarrayEurosurveillance, 2013
- Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virusJournal of Clinical Virology, 2009
- Profiling the humoral immune response to infection by using proteome microarrays: High-throughput vaccine and diagnostic antigen discoveryProceedings of the National Academy of Sciences of the United States of America, 2005