Novel Mutation of Exon 5 Prolactin Gene in IPB-D1 Chicken
Open Access
- 14 December 2020
- journal article
- research article
- Published by Indonesian Center for Animal Research and Development (ICARD) in Jurnal Ilmu Ternak dan Veteriner
- Vol. 25 (4), 173-181
- https://doi.org/10.14334/jitv.v25i4.2525
Abstract
The prolactin gene (PRL) is a gene that controls the incubation and egg production in laying chickens. The nature of incubation will reduce egg production and disrupt the reproductive system in local chickens. The purpose of this study was to identify the polymorphism of prolactin genes in IPB-D1 chickens using the direct sequencing method. The polymorphism of the exon 5 prolactin gene was carried out on 46 samples of IPB-D1 chicken DNA which was a collection of the Division of Animal Genetics and Breeding, Faculty of Animal Science IPB. DNA sequences as a reference for designing exon 5 primers were obtained from the National Center for Biotechnology Information (NCBI) with the GenBank access code: AF288765.2. DNA extraction was carried out using the phenol-chloroform technique. DNA amplification resulted in a PCR product with a size of 557 bp. In this study, the genotype frequency, allele frequency, heterozygosity value and Hardy-Weinberg equilibrium were calculated. The results of the study found 5 SNPs in exon 5, namely g.7823A>G, g.7835A>G, g.7886T>A, g.8052T>C, and g.8069T>C. All SNPs are polymorphic and in Hardy-Weinberg equilibrium except g.8052T>C. The g.7823A>G, g.7835A>G, g.8052T>C SNPs are synonymous mutations that do not change amino acids, while the g.7886T>A and g.8069T>C SNPs are non-synonymous that change amino acids. Both g.7886T>A and g.8069T>C SNPs are potential as a marker assisted selection for the characteristics of egg production in IPB-D1 chickens.Keywords
This publication has 12 references indexed in Scilit:
- DIGESTIBLE VALINE REQUIREMENTS IN LOW-PROTEIN DIETS FOR BROILERS CHICKSBrazilian Journal of Poultry Science, 2016
- Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail LinesAsian-Australasian Journal of Animal Sciences, 2016
- Analysing the Effect of Mutation on Protein Function and Discovering Potential Inhibitors of CDK4: Molecular Modelling and Dynamics StudiesPLOS ONE, 2015
- Genome measures used for quality control are dependent on gene function and ancestryBioinformatics, 2014
- Synonymous Mutations Frequently Act as Driver Mutations in Human CancersCell, 2014
- Analysis of the genetic effects of prolactin gene polymorphisms on chicken egg productionMolecular Biology Reports, 2012
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reactionBMC Bioinformatics, 2012
- A pathway map of prolactin signalingJournal of Cell Communication and Signaling, 2012
- Understanding the contribution of synonymous mutations to human diseaseNature Reviews Genetics, 2011
- Review of Three DNA Analysis Applications for Use in the Microbiology or Genetics ClassroomJournal of Microbiology & Biology Education, 2010