Determination of Genetic Diversity Using 15 Simple Sequence Repeats Markers in Long Term Selected Japanese Quail Lines
Open Access
- 22 April 2016
- journal article
- Published by Asian Australasian Association of Animal Production Societies in Asian-Australasian Journal of Animal Sciences
- Vol. 29 (12), 1696-1701
- https://doi.org/10.5713/ajas.15.0940
Abstract
Japanese quail is still used as a model for poultry research because of their usefulness as laying, meat, and laboratory animals. Microsatellite markers are the most widely used molecular markers, due to their relative ease of scoring and high levels of polymorphism. The objective of the research was to determine genetic diversity and population genetic structures of selected Japanese quail lines (high body weight 1 [HBW1], HBW2, low body weight [LBW], and layer [L]) throughout 15th generations and an unselected control (C). A total of 69 individuals from five quail lines were genotyped by fifteen microsatellite markers. When analyzed profiles of the markers the observed (Ho) and expected (He) heterozygosity ranged from 0.04 (GUJ0027) to 0.64 (GUJ0087) and 0.21 (GUJ0027) to 0.84 (GUJ0037), respectively. Also, Ho and He were separated from 0.30 (L and LBW) to 0.33 (C and HBW2) and from 0.52 (HBW2) to 0.58 (L and LBW), respectively. The mean polymorphic information content (PIC) ranged from 0.46 (HBW2) to 0.52 (L). Approximately half of the markers were informative (PIC≥0.50). Genetic distances were calculated from 0.09 (HBW1 and HBW2) to 0.33 (C and L). Phylogenetic dendrogram showed that the quail lines were clearly defined by the microsatellite markers used here. Bayesian model-based clustering supported the results from the phylogenetic tree. These results reflect that the set of studied markers can be used effectively to capture the magnitude of genetic variability in selected Japanese quail lines. Also, to identify markers and alleles which are specific to the divergence lines, further generations of selection are required.Keywords
Funding Information
- Akdeniz University
- The Scientific and Technological Research Council of Turkey
This publication has 12 references indexed in Scilit:
- Cost-effective development of highly polymorphic microsatellite in Japanese quail facilitated by next-generation sequencingAnimal Genetics, 2014
- Assessing paternity in japanese quails (Coturnix japonica) using microsatellite markers - inferences for its mating system and reproductive successBrazilian Journal of Poultry Science, 2013
- STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno methodConservation Genetics Resources, 2011
- Evaluation of diversity between different Spanish chicken breeds, a tester line, and a White Leghorn population based on microsatellite markersPoultry Science, 2009
- Fast, cost-effective development of species-specific microsatellite markers by genomic sequencingBioTechniques, 2009
- Genetic Diversity of Wild Quail in China Ascertained with Microsatellite DNA MarkersAsian-Australasian Journal of Animal Sciences, 2007
- Inbreeding in Japanese Quail Estimated by Pedigree and Microsatellite AnalysesJournal of Heredity, 2007
- Revising how the computer program cervus accommodates genotyping error increases success in paternity assignmentMolecular Ecology, 2007
- Detecting the number of clusters of individuals using the software structure: a simulation studyMolecular Ecology, 2005
- Cluster Analysis of 12 Chinese Native Chicken Populations Using Microsatellite MarkersAsian-Australasian Journal of Animal Sciences, 2004