TGF-β regulates isoform switching of FGF receptors and epithelial-mesenchymal transition

Abstract

The epithelial–mesenchymal transition (EMT) is a crucial event in wound healing, tissue repair, and cancer progression in adult tissues. Here, we demonstrate that transforming growth factor (TGF)‐β induced EMT and that long‐term exposure to TGF‐β elicited the epithelial–myofibroblastic transition (EMyoT) by inactivating the MEK‐Erk pathway. During the EMT process, TGF‐β induced isoform switching of fibroblast growth factor (FGF) receptors, causing the cells to become sensitive to FGF‐2. Addition of FGF‐2 to TGF‐β‐treated cells perturbed EMyoT by reactivating the MEK‐Erk pathway and subsequently enhanced EMT through the formation of MEK‐Erk‐dependent complexes of the transcription factor δEF1/ZEB1 with the transcriptional corepressor CtBP1. Consequently, normal epithelial cells that have undergone EMT as a result of combined TGF‐β and FGF‐2 stimulation promoted the invasion of cancer cells. Thus, TGF‐β and FGF‐2 may cooperate with each other and may regulate EMT of various kinds of cells in cancer microenvironment during cancer progression.