Optogenetic probing of functional brain circuitry
- 3 December 2010
- journal article
- review article
- Published by Wiley in Experimental Physiology
- Vol. 96 (1), 26-33
- https://doi.org/10.1113/expphysiol.2010.055731
Abstract
Recently developed optogenetic technologies offer the promise of high-speed mapping of brain circuitry. Genetically targeted light-gated channels and pumps, such as channelrhodopsins and halorhodopsin, allow optical control of neuronal activity with high spatial and temporal resolution. Optogenetic probes of neuronal activity, such as Clomeleon and Mermaid, allow light to be used to monitor the activity of a genetically defined population of neurons. Combining these two complementary sets of optogenetic probes will make it possible to perform all-optical circuit mapping. Owing to the improved efficiency and higher speed of data acquisition, this hybrid approach should enable high-throughput mapping of brain circuitry.Keywords
This publication has 50 references indexed in Scilit:
- Effect of Voltage Sensitive Fluorescent Proteins on Neuronal ExcitabilityBiophysical Journal, 2009
- Characterization of Engineered Channelrhodopsin Variants with Improved Properties and KineticsBiophysical Journal, 2009
- Imaging activity of neuronal populations with new long-wavelength voltage-sensitive dyesBrain Cell Biology, 2008
- Genetically encoded fluorescent sensors of membrane potentialBrain Cell Biology, 2008
- eNpHR: a Natronomonas halorhodopsin enhanced for optogenetic applicationsBrain Cell Biology, 2008
- Improved expression of halorhodopsin for light-induced silencing of neuronal activityBrain Cell Biology, 2008
- Imaging synaptic inhibition throughout the brain via genetically targeted ClomeleonBrain Cell Biology, 2008
- In Vivo Light-Induced Activation of Neural Circuitry in Transgenic Mice Expressing Channelrhodopsin-2Neuron, 2007
- Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, ClomeleonBrain Cell Biology, 2006
- Clathrin-Mediated Endocytosis Is the Dominant Mechanism of Vesicle Retrieval at Hippocampal SynapsesNeuron, 2006