Genetically encoded fluorescent sensors of membrane potential
Open Access
- 5 August 2008
- journal article
- review article
- Published by Springer Science and Business Media LLC in Brain Cell Biology
- Vol. 36 (1-4), 53-67
- https://doi.org/10.1007/s11068-008-9026-7
Abstract
Imaging activity of neurons in intact brain tissue was conceived several decades ago and, after many years of development, voltage-sensitive dyes now offer the highest spatial and temporal resolution for imaging neuronal functions in the living brain. Further progress in this field is expected from the emergent development of genetically encoded fluorescent sensors of membrane potential. These fluorescent protein (FP) voltage sensors overcome the drawbacks of organic voltage sensitive dyes such as non-specificity of cell staining and the low accessibility of the dye to some cell types. In a transgenic animal, a genetically encoded sensor could in principle be expressed specifically in any cell type and would have the advantage of staining only the cell population determined by the specificity of the promoter used to drive expression. Here we critically review the current status of these developments.Keywords
This publication has 48 references indexed in Scilit:
- Subunit organization and functional transitions in Ci-VSPNature Structural & Molecular Biology, 2007
- In vivo calcium imaging from genetically specified target cells in mouse cerebellumNeuroImage, 2007
- Optical Recording of Action Potentials and Other Discrete Physiological Events: A Perspective from Signal Detection TheoryPhysiology, 2007
- Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cellsJournal of Neuroscience Methods, 2006
- A Voltage Sensor-Domain Protein Is a Voltage-Gated Proton ChannelScience, 2006
- Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imagingNature Methods, 2006
- A voltage-gated proton-selective channel lacking the pore domainNature, 2006
- Optical probing of neuronal circuit dynamics: genetically encoded versus classical fluorescent sensorsTrends in Neurosciences, 2006
- Genetic Targeting of Individual Cells with a Voltage‐Sensitive Dye through Enzymatic Activation of Membrane BindingChemBioChem, 2006
- Millisecond-timescale, genetically targeted optical control of neural activityNature Neuroscience, 2005