Comparison of lipopolysaccharide and outer membrane protein-lipopolysaccharide extracts in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis infection
- 1 May 1986
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 23 (5), 938-942
- https://doi.org/10.1128/jcm.23.5.938-942.1986
Abstract
Brucella ovis hot saline extracts and petroleum ether-chloroform-phenol lipopolysaccharide were compared in an enzyme-linked immunosorbent assay for the diagnosis of B. ovis ram epididymitis. Hot saline extracts detected greater numbers of infected rams. Chemical characterization of the antigens showed that, although both contained lipopolysaccharide, hot saline extracts also contained outer membrane proteins. These proteins were active as antigens in Western blot tests with sera of infected rams, and therefore they explained the better diagnostic results obtained with hot saline extracts. However, compared with lipopolysaccharide, hot saline extracts showed a higher degree of cross-reactivity with sera from smooth B. melitensis-infected animals. This observation might be explained by the presence of B. ovis outer membrane proteins in hot saline extracts which lack the specificity necessary for serological identification of the Brucella species present.Keywords
This publication has 23 references indexed in Scilit:
- Comparison of an enzyme-linked immunospecific assay (ELISA) with the cold complement fixation test for the serodiagnosis ofBrucella ovisinfectionNew Zealand Veterinary Journal, 1984
- Comparison of the enzyme-linked immunosorbent assay and complement fixation test for detecting Brucella ovis antibodies in sheepVeterinary Record, 1983
- Comparison of different antigenic preparations for the detection of ovine serum antibodies against Brucella ovis by ELISAAustralian Veterinary Journal, 1983
- Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteriaBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1983
- A dot-immunobinding assay for monoclonal and other antibodiesAnalytical Biochemistry, 1982
- Identification of outer membrane proteins, including known lymphocyte mitogens, as the endotoxin protein of Escherichia coli 0111.The Journal of Immunology, 1981
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A New Method for the Extraction of R LipopolysaccharidesJBIC Journal of Biological Inorganic Chemistry, 1969
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951