A dot-immunobinding assay for monoclonal and other antibodies
- 1 January 1982
- journal article
- Published by Elsevier BV in Analytical Biochemistry
- Vol. 119 (1), 142-147
- https://doi.org/10.1016/0003-2697(82)90677-7
Abstract
A procedure for the assay of antibodies in sera based on the application of the antigen as a spot to nitrocellulose filters is described. The method has the merit of being simpler in operation and more sensitive than comparable existing procedures. Applications for screening supernatants of hybridomas making monoclonal antibodies, and the use of such antibodies in the determination of the tissue distribution of the corresponding antigens, are described. An application for the screening of human pathological sera for multiple antibodies in one operation is also described.Keywords
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