Colonization of hospital water systems by Legionella pneumophila, Pseudomonas aeroginosa, and Acinetobacter in ICU wards of Tehran hospitals
Open Access
- 1 January 2012
- journal article
- research article
- Published by Medknow in Indian Journal of Pathology and Microbiology
- Vol. 55 (3), 352-356
- https://doi.org/10.4103/0377-4929.101743
Abstract
Background: Nosocomial infection caused by non-Enterobacteriaceae gram negative bacteria (GNB-NE) is increasing in intensive care units (ICU). Aim: The objective of this study was to determine whether potable water in ICU wards at Tehran hospitals is contaminated with L. pneomophila, P. aeroginosa and Acinetobacter spp. Materials and Methods: A total of 52 water samples from shower bath and taps water in seven hospitals of Tehran were collected. The water sample concentrated by filtering through millipore cellulose filters and cultured on BCYE agar and tryptic soya agar media. The presence of Legionella pneumophila was confirmed by real time PCR assay using primers-probe designed for the mip gene. Results: Legionella pneumophila, Pseudomonas aeroginosa and Acinetobacter were isolated from 5 (9.6%), 6 (11.4%) and 1 (1.8%) of the hospital water systems, respectively. This study demonstrated the presence of Legionella, Pseudomonas and Acinetobacter in water system in ICU wards of different hospitals in Tehran. Conclusions: Hot water from shower heads could be a potential source of infection for Legionella pneumophila. Water was also proved to contain Pseudomonas aeruginonsa, the main GNB-NE causing nosocomila pneumonia at Tehran hospitals. Care should be taken concerning cleanliness and decontamination of water supplies at ICUs for pathogenic organisms.Keywords
This publication has 11 references indexed in Scilit:
- Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from clinical samples of CAPActa Microbiologica Et Immunologica Hungarica, 2012
- Assessment of biofilm formation in device-associated clinical bacterial isolates in a tertiary level hospitalIndian Journal of Pathology and Microbiology, 2011
- Direct detection of Pseudomonas aeruginosa from patients with healthcare associated pneumonia by real time PCRInfection, Genetics and Evolution, 2010
- Fluorescence acquisition during hybridization phase in quantitative real-time PCR improves specificity and signal-to-noise ratioBioTechniques, 2008
- The high prevalence of Legionella pneumophila contamination in hospital potable water systems in Taiwan: implications for hospital infection control in AsiaInternational Journal of Infectious Diseases, 2008
- Legionella spp. and Legionnaires' diseaseJournal of Infection, 2008
- Colonization ofLegionellaSpecies in Hotel Water Systems in TurkeyJournal of Travel Medicine, 2007
- Ecology of Pseudomonas aeruginosa in the intensive care unit and the evolving role of water outlets as a reservoir of the organismAmerican Journal of Infection Control, 2005
- Faucets as a reservoir of endemic Pseudomonas aeruginosa colonization/infections in intensive care unitsIntensive Care Medicine, 2004
- Detection of Legionella pneumophila by Real-Time PCR for the mip GeneJournal of Clinical Microbiology, 2003