Fluorescence Acquisition During Hybridization Phase in Quantitative Real-Time PCR Improves Specificity and Signal-to-Noise Ratio
Open Access
- 1 December 2008
- journal article
- Published by Taylor & Francis Ltd in BioTechniques
- Vol. 45 (6), 625-634
- https://doi.org/10.2144/000112994
Abstract
Quantitative real-time PCR (qPCR) is a standard method used for quantification of specific gene expression. This utilizes either dsDNA binding dyes or probe based chemistry. While dsDNA binding dye...This publication has 10 references indexed in Scilit:
- Real-time quantification of microRNAs by stem-loop RT-PCRNucleic Acids Research, 2005
- Mathematics of quantitative kinetic PCR and the application of standard curvesNucleic Acids Research, 2003
- A stochastic model on DNA renaturation kineticsBiophysical Chemistry, 2003
- Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) dataNeuroscience Letters, 2003
- Improved mRNA Quantitation in LightCycler RT-PCRInternational Archives of Allergy and Immunology, 2003
- Ribosomal 18S RNA Prevails over Glyceraldehyde-3-Phosphate Dehydrogenase and β-Actin Genes as Internal Standard for Quantitative Comparison of mRNA Levels in Invasive and Noninvasive Human Melanoma Cell SubpopulationsAnalytical Biochemistry, 2001
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Bias in Estimations of DNA Content by Competitive Polymerase Chain ReactionAnalytical Biochemistry, 2000
- Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCRJournal of Biochemical and Biophysical Methods, 2000
- Quantitative RT-PCR: Pitfalls and PotentialBioTechniques, 1999