Regional survey of CTX-M-type extended-spectrum -lactamases among Enterobacteriaceae reveals marked heterogeneity in the distribution of the ST131 clone
Open Access
- 23 December 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Antimicrobial Chemotherapy
- Vol. 66 (3), 505-511
- https://doi.org/10.1093/jac/dkq482
Abstract
To establish the prevalence and diversity of clinically significant extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae harbouring blaCTX-M in the West Midlands region of the UK. During a 2 month period, 370 consecutive, non-duplicate isolates were collected from 13 laboratories. Isolates were screened for the presence of blaCTX-M by multiplex PCR and genotyped using denaturing HPLC (DHPLC). Clonal relationships were studied by PFGE and O25b-ST131 Escherichia coli were identified by PCR. Two hundred and ninety-four out of 345 ESBL-producing isolates (85.2%) carried blaCTX-M. CTX-M group 1 enzymes were expressed in 284 (96.6%) isolates, with the other 10 carrying group 9, 2 and 25/26 genes. All group 1 isolates had blaCTX-M-15 DHPLC profiles. The blaCTX-ME. coli were split into 23 PFGE clusters. The largest cluster (RE1) was indistinguishable from the previously described strain A and all but one harboured blaCTX-M-15. A total of 66% of E. coli were O25b-ST131 positive. The CTX-M-15-producing RE1 clone (strain A) is the predominant clone in the West Midlands. This clone has spread throughout the region since its emergence in an outbreak 3 years earlier. Most, but not all, RE1 isolates belong to the O25b-ST131 lineage, providing further evidence that this lineage plays a pivotal role in the clonal dispersal of CTX-M-15-producing Enterobacteriaceae. Strain A was found to be considerably more heterogeneous than when first described and has acquired greater resistance to gentamicin. Approximately one-third of CTX-M producers represented a wide variety of unrelated strains. The study shows the rapid spread and diversification of CTX-M-producing Enterobacteriaceae over a 3 year period.Keywords
This publication has 24 references indexed in Scilit:
- CTX-M enzymes are the predominant extended-spectrum β-lactamases produced by Enterobacteriaceae in IrelandJournal of Antimicrobial Chemotherapy, 2009
- Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strainsJournal of Antimicrobial Chemotherapy, 2009
- Molecular epidemiology of Escherichia coli producing extended-spectrum β-lactamases in Lugo (Spain): dissemination of clone O25b:H4-ST131 producing CTX-M-15Journal of Antimicrobial Chemotherapy, 2009
- Nationwide survey of extended-spectrum β-lactamase-producing Enterobacteriaceae in the French community settingJournal of Antimicrobial Chemotherapy, 2009
- Rapid Genotyping of CTX-M Extended-Spectrum β-Lactamases by Denaturing High-Performance Liquid ChromatographyAntimicrobial Agents and Chemotherapy, 2007
- Resistance of strains producing extended-spectrum β-lactamases and genotype distribution in ChinaJournal of Infection, 2007
- Prevalence and mechanisms of cephalosporin resistance in Enterobacteriaceae in London and South-East EnglandJournal of Antimicrobial Chemotherapy, 2006
- Rapid and simple detection of bla CTX−M genes by multiplex PCR assayJournal of Medical Microbiology, 2005
- CTX-M: changing the face of ESBLs in the UKJournal of Antimicrobial Chemotherapy, 2005
- Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum β-lactamases in the UKJournal of Antimicrobial Chemotherapy, 2004