Rapid and simple detection of bla CTX−M genes by multiplex PCR assay
- 1 December 2005
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 54 (12), 1183-1187
- https://doi.org/10.1099/jmm.0.46160-0
Abstract
A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of blaCTX−M genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified blaCTX−M genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.Keywords
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