Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82
Open Access
- 1 April 1994
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 176 (8), 2439-2443
- https://doi.org/10.1128/jb.176.8.2439-2443.1994
Abstract
A 25-kb DNA SalI fragment cloned from the chromosomal DNA of Pseudomonas putida OUS82, which utilizes phenanthrene (Phn+) and naphthalene (Nah+), carried all of the genes necessary for upper naphthalene catabolism. Cosmid recombinant pIP7 complemented both the Nah- and Phn- defects of OUS8211 (Trp-Nah-Phn-Sal+[salicylate utilizing]Hna+[1-hydroxy-2-naphthoate utilizing]) and only the Phn- defect of OUS8212 (Trp-Nah-Phn-Sal-Hna+). The results indicate that strain OUS82 uses different pathways after o-hydroxycarboxylic aromatics in the catabolism of naphthalene and phenanthrene.Keywords
This publication has 22 references indexed in Scilit:
- Sequences of genes encoding naphthalene dioxygenase in Pseudomonas putida strains G7 and NCIB 9816-4Gene, 1993
- Isolation and characterization of altered plasmids in mutant strains of Pseudomonasputida NCIB 9816Biochemical and Biophysical Research Communications, 1989
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of IndigoScience, 1983
- Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutantsGene, 1982
- The cometabolism of 1- and 2-chloronaphthalene by pseudomonadsCanadian Journal of Microbiology, 1982
- Isolation and some properties of NAD-linked 2-carboxybenzaldehyde dehydrogenase in Alcaligenes faecalis AFK 2 grown on phenanthrene.The Journal of General and Applied Microbiology, 1981
- The Catabolism of Phenanthrene and Naphthalene by BacteriaJournal of General Microbiology, 1978
- Oxidation of the Carcinogens Benzo[ a ]pyrene and Benzo[ a ]anthracene to Dihydrodiols by a BacteriumScience, 1975
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969