Expression of Naphthalene Oxidation Genes in Escherichia coli Results in the Biosynthesis of Indigo
- 14 October 1983
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 222 (4620), 167-169
- https://doi.org/10.1126/science.6353574
Abstract
A fragment of plasmid NAH7 from Pseudomonas putida PpG7 has been cloned and expressed in Escherichia coli HB101. Growth of the recombinant Escherichia coli in nutrient medium results in the formation of indigo. The production of this dye is increased in the presence of tryptophan or indole. Several bacteria that oxidize aromatic hydrocarbons to cis-dihydrodiols also oxidize indole to indigo. The results suggest that indigo formation is due to the combined activities of tryptophanase and naphthalene dioxygenase.This publication has 15 references indexed in Scilit:
- Initial reactions in the oxidation of naphthalene by Pseudomonas putidaBiochemistry, 1975
- Oxidation of biphenyl by a Beijerinckia speciesBiochemical and Biophysical Research Communications, 1973
- Oxidative degradation of aromatic hydrocarbons by microorganisms. III. Formation of (+)-cis-2,3-dihydroxy-1-methyl-4,6-cyclohexadiene from toluene by Pseudomonas putidaBiochemistry, 1970
- Oxidative degradation of aromatic hydrocarbons by microorganisms. I. Enzymic formation of catechol from benzeneBiochemistry, 1968
- The bacterial oxidation of indoleBiochimica et Biophysica Acta (BBA) - General Subjects, 1968
- Oxidation of Indole to Indigotin by Pseudomonas indoloxidans*The Journal of Biochemistry, 1965
- THE BACTERIAL DEGRADATION OF CATECHOLBiochemical Journal, 1965
- Oxidative metabolism of naphthalene by soil pseudomonads. The ring-fission mechanismBiochemical Journal, 1964
- Divergent Pathways of Indole Metabolism in Chromobacterium violaceumNature, 1962
- The formation of indigotin from indol by soil bacteriaProceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character, 1928