Perisynaptic Localization of δ Subunit-Containing GABAAReceptors and Their Activation by GABA Spillover in the Mouse Dentate Gyrus

Abstract

In cerebellar granule cells, δ subunit-containing GABA_Areceptors are found exclusively at extrasynaptic sites, but their subcellular distribution in other brain areas is poorly understood. We examined the anatomical localization and physiological activation of these receptors in adult mouse dentate gyrus granule cells. Immunocytochemistry revealed a high density of δ subunits in the molecular layer and a much lower density in the cell body layer. At the ultrastructural level, immunogold-labeled δ subunits were found at the edge of symmetric synapses on granule cell dendrites. Functional correlates of this perisynaptic localization were obtained by comparing inhibitory responses in δ subunit-deficient (δ-/-) and wild-type (wt) mice. In whole-cell recordings at 22°C, the weighted decay time constants (τ_w) of spontaneous IPSCs (sIPSCs) were significantly longer in wt mice but were similar at 34°C, reflecting the role of temperature-dependent GABA uptake in shaping sIPSC decay. IPSCs evoked by minimal stimulation (eIPSCs) near the somata had similar τ_win δ-/- and wt mice, but eIPSCs elicited from dendritic sites decayed significantly more slowly in wt mice, consistent with a higher density of δ subunit-containing receptors in the molecular layer. The τ_wof dendritic eIPSCs of wt mice were shortened by ZnCl₂(10 μm), reflecting the high Zn²⁺sensitivity of δ subunit-containing GABA_Areceptors, and were prolonged by the GAT-1 GABA transporter inhibitor NO711 (10 μm). Our results demonstrate a perisynaptic localization of δ subunit-containing GABA_Areceptors and indicate that these receptors can be activated by GABA overspill in the molecular layer.