Characterization of a filovirus (Měnglà virus) from Rousettus bats in China
Top Cited Papers
- 7 January 2019
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Microbiology
- Vol. 4 (3), 390-395
- https://doi.org/10.1038/s41564-018-0328-y
Abstract
Filoviruses, especially Ebola virus (EBOV) and Marburg virus (MARV), are notoriously pathogenic and capable of causing severe haemorrhagic fever diseases in humans with high lethality(1/2). The risk of future outbreaks is exacerbated by the discovery of other bat-borne filoviruses of wide genetic diversity globally(3-5). Here we report the characterization of a phylogenetically distinct bat filovirus, named Mengla virus (MLAV). The coding-complete genome of MLAV shares 32-54% nucleotide sequence identity with known filoviruses. Phylogenetic analysis places this new virus between EBOV and MARV, suggesting the need for a new genus taxon. Importantly, despite the low amino acid sequence identity (22-39%) of the glycoprotein with other filoviruses, MLAV is capable of using the Niemann-Pick C1 (NPC1) as entry receptor. MLAV is also replication-competent with chimeric MLAV mini-genomes containing EBOV or MARV leader and trailer sequences, indicating that these viruses are evolutionally and functionally closely related. Finally, MLAV glycoprotein-typed pseudo-types transduced cell lines derived from humans, monkeys, dogs, hamsters and bats, implying a broad species cell tropism with a high risk of interspecies spillover transmission.Keywords
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