The Glycoproteins of All Filovirus Species Use the Same Host Factors for Entry into Bat and Human Cells but Entry Efficiency Is Species Dependent
Open Access
- 22 February 2016
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 11 (2), e0149651
- https://doi.org/10.1371/journal.pone.0149651
Abstract
Ebola and marburgviruses, members of the family Filoviridae, can cause severe hemorrhagic fever in humans. The ongoing Ebola virus (EBOV) disease epidemic in Western Africa claimed more than 11,300 lives and was associated with secondary cases outside Africa, demonstrating that filoviruses pose a global health threat. Bats constitute an important natural reservoir of filoviruses, including viruses of the recently identified Cuevavirus genus within the Filoviridae family. However, the interactions of filoviruses with bat cells are incompletely understood. Here, we investigated whether filoviruses employ different strategies to enter human and bat cells. For this, we examined host cell entry driven by glycoproteins (GP) from all filovirus species into cell lines of human and fruit bat origin. We show that all GPs were able to mediate entry into human and most fruit bat cell lines with roughly comparable efficiency. In contrast, the efficiency of entry into the cell line EidNi/41 derived from a straw-colored fruit bat varied markedly between the GPs of different filovirus species. Furthermore, inhibition studies demonstrated that filoviruses employ the same host cell factors for entry into human, non-human primate and fruit bat cell lines, including cysteine proteases, two pore channels and NPC1 (Niemann-Pick C1 molecule). Finally, processing of GP by furin and the presence of the mucin-like domain in GP were dispensable for entry into both human and bat cell lines. Collectively, these results show that filoviruses rely on the same host cell factors for entry into human and fruit bat cells, although the efficiency of the usage of these factors might differ between filovirus species.This publication has 76 references indexed in Scilit:
- The Spike Protein of the Emerging Betacoronavirus EMC Uses a Novel Coronavirus Receptor for Entry, Can Be Activated by TMPRSS2, and Is Targeted by Neutralizing AntibodiesJournal of Virology, 2013
- Lectin-Dependent Enhancement of Ebola Virus Infection via Soluble and Transmembrane C-type Lectin ReceptorsPLOS ONE, 2013
- Host Cell Factors in Filovirus Entry: Novel Players, New InsightsViruses, 2012
- Comparative Analysis of Ebola Virus Glycoprotein Interactions With Human and Bat CellsThe Journal of Infectious Diseases, 2011
- Small molecule inhibitors reveal Niemann–Pick C1 is essential for Ebola virus infectionNature, 2011
- Ebola virus entry requires the cholesterol transporter Niemann–Pick C1Nature, 2011
- T-cell immunoglobulin and mucin domain 1 (TIM-1) is a receptor for Zaire Ebolavirus and Lake Victoria MarburgvirusProceedings of the National Academy of Sciences of the United States of America, 2011
- A Small-Molecule Oxocarbazate Inhibitor of Human Cathepsin L Blocks Severe Acute Respiratory Syndrome and Ebola Pseudotype Virus Infection into Human Embryonic Kidney 293T cellsMolecular Pharmacology, 2010
- α 5 β 1 -Integrin controls ebolavirus entry by regulating endosomal cathepsinsProceedings of the National Academy of Sciences of the United States of America, 2009
- Tyro3 Family-Mediated Cell Entry of Ebola and Marburg VirusesJournal of Virology, 2006